Table 5: Comparison of the current study with the literature data [1820].

Current studyRef. [19]Ref. [18]Ref. [20]

SpeciesMouseMouseMouseRat
SampleLiver microsomeLiver microsomeLiver microsomeER, Golgi
PretreatmentNonePhenobarbitalPhenobarbital or 3-methylcholanthreneNone
Sample preparationSubfractionated by differential ultra-centrifugation + sucrose gradient centrifugation + Na2CO3Subfractionated by differential ultra-centrifugationDifferential ultracentrifugationSubfractionated by differential ultra-centrifugation + density gradient centrifugation
Protein separation2DE, 1DE1DE2DE1DE
1D/2D-Gel StainCoomassie brilliant blue (2DE; 1DE)Silver stain
Protein identificationMS/MSMS/MSPMFMS/MS
Protein spots on 2D-Gel5141100
Proteins identified in 2D-Gel183139
Proteins identified in 1DE99519832 (ER)
Proteins identified in 2-D LC1410
Proteins identified in 3-D LC3703
Total identified proteins2594142Unspecified832 (ER)
Membrane proteins2DE: 41 (41/183 = 22%)
1DE: 54 (54/99 = 55%)
Total: 62 (62/259 = 24%)
1DE: 138 (138/519 = 27%)
2-D LC: 259 (259/1410 = 21%)
3-D LC: 659 (659/3703 = 18%)
Unspecified183 (183/832 = 22%)
Protein superfamily   
 P450 family members1029211
 Ribosomal proteins1316Unspecified45
 UDP glycosyltransferases, UGTs68Unspecified3
 Tubulins115Unspecified2
 Short-chain  dehydrogenase/reductase329Unspecified56
 Protein disulfide isomerase24Unspecified1