|
| Authors/year | Specimen | Quantitative approach | Sample
preparation | Outcomes |
|
| Yang et al. 2011 [46] | Urine
54 bladder cancer patients, 46 controls | Label-free—spectral count | NIL | Quantified 265 glycoproteins. alpha-1-antitrypsin, 74% sensitivity and 80% specificity for bladder cancer patients. |
| Quintana et al. 2009 [47] | Urine
39 patients kidney chronic allograft dysfunction, 32 controls | Label-free—peak peptide intensity | SCX using
magnetic beads | Peptides from uromodulin and kininogen significantly elevated in control compared to CAD patients. |
| Hanas et al. 2008 [48] | Serum
13 pancreatic adenocarcinoma patients, 12 healthy controls
| Label-free—peak peptide intensity | NIL | Quantified 20 low-mass serum peaks. Bootstrap analysis showed peaks could differentiate cancer from control sera with 95% accuracy. |
| Xue et al. 2010 [49] | Cell lysates
Primary and lymph node metastatic cell lines. 1 patient. | Label-free—peak peptide intensity | NIL | 145 differential proteins. Western blot and ROC curve analysis confirmed that 2 specific proteins could predict colorectal cancer metastasis. |
Besson et al. 2011
[50] | Colorectal cancer tissue
28 colorectal frozen tissue samples
| Stable isotope labeling—iTRAQ | Peptide
OFFGEL
fractionation | 555 proteins with significant fold change between different cancer stages. Identified a candidate with increased abundance in adenomas and early stage colorectal cancer. |
| Bondar et al. 2007 [51] | Serum
6 healthy male, 20 nonmalignant prostate biopsy patients, 26 malignant prostate cancer patients | Stable isotope labeling | NIL | Higher abundance of Zn-α2 glycoprotein (ZAG) in prostate cancer patients than nonmalignant prostate disease patients and healthy controls. |
| Chaerkady et al. 2008 [52] | Liver tissue
55 samples of hepatocellular carcinoma, 20 samples of adjacent noncancer tissues | Stable isotope labeling—iTRAQ | SCX | 59 proteins increased in abundant, 92 proteins were less abundant in HCC compared to normal tissue. 12 proteins further validated using immunohistochemical labeling. |
Dayon et al. 2008
[53] | Cerebrospinal fluid
4 postmortem CSF patients, 4 antemortem CSF from living healthy controls | Stable isotope labeling—tandem mass tag isobaric labeling | Immunoaffinity depletion of 6
most abundant
proteins and SCX | 78 proteins more abundant in postmortem samples compared to antemortem. |
|
