An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells
Figure 2
VPS4B downregulation decreases the expression of fatty acid synthase (FASN) in SKBR3_shVPS4B cells. Representative MS1 spectra of fatty acid synthase peptide SLLVNPEGPTLMR in SKBR3 (a) and SKBR3_shVPS4B cells (b). The relative abundance of unlabeled peptides (, labeled in blue) or labeled peptides (, label in red), expressed as , was calculated by our in-house software, IsoQuant [38]. VPS4B downregulation increased the degradation rate of the SLLVNPEGPTLMR FASN peptide (c) and decreased its synthesis rate (d) in SKBR3 cells, which was related to the decrease of its relative abundance after EGF treatment (e).
