Additions to the Human Plasma Proteome via a Tandem MARS Depletion iTRAQ-Based Workflow
Figure 3
(a) The distribution of CV values for (grey rectangular), (shaded rectangular), and (black rectangular) for proteins quantified in each of the three independent experiments ( proteins). The cumulative frequency of proteins with specific CV values for (dashed square), (dashed triangle), and (dashed circle) are shown. CV values are given as fractional values. The total peak intensity is represented by I. (b) Plot of the mean CV values for reporter ion ratios relative to reference channel 114 as a function of the number of spectral counts identified for each protein. Only proteins identified in all three workflow replicates are represented in this plot. (c) Power analysis for iTRAQ-based quantitative platform whereby fold change values are plotted for a given number of biological replicates as a function of the number of technical replicates (i.e., to 4). The power and significance level values were set to 80% and 0.05, respectively.
