Quantitative Proteomics via High Resolution MS Quantification: Capabilities and Limitations

Figure 2

Dilutional linearity of yeast enolase titrated into a TFA/water background. Enolase quantification using the average of all peptides (black), average of model selected peptides (orange), and average of dilution optimal selected peptides (blue). Deviation from the theoretical dilution line for the model selected and all peptide curves is attributed to nonspecific binding with a TFA/water matrix.