Issues and Applications in Label-Free Quantitative Mass Spectrometry
Figure 2
Multiple MS/MS scans of a single peptide. (a) The peptide is eluted from an LC column. The scan time points for the last MS/MS scan before the peak and the first two MS/MS scans after the peak are label in red. (b) At scan #4039, an MS/MS scan of the precursor ion m/z 786.09 is performed almost one-half minute before the apex of the peptide peak. (c) At scan #4243, the first repeat MS/MS scan of the precursor ion m/z 786.09 appears two minutes later and is performed almost one-and-a-half minutes after the apex of the peptide peak. (d) All the MS/MS scans of the peptide are listed. Most of them appear far behind the peptide peak.
