Label-Free Quantitation and Mapping of the ErbB2 Tumor Receptor by Multiple Protease Digestion with Data-Dependent (MS1) and Data-Independent (MS2) Acquisitions
Figure 2
Reproducibility assessment of MS1 Filtering and digestion of ErbB2 by multiple proteases. (a) Percent coefficient of variation (%CV) of individual ErbB2 peptide samples was determined by MS1 Filtering. Each individually digested sample was analyzed with data-dependent acquisition in triplicate, MS replicates, and each enzyme digestion was performed in triplicate, process replicates. The difference between process and MS replicates represents the added peptide variability due to enzyme digestion. (b) Box and whisker plot of the %CV of MS and process replicates for ErbB2 peptides detected in the four enzyme conditions assessed. (c) Scatter plot of the MS replicate and process replicate %CV for each ErbB2 peptide detected.
