Enhanced Photosynthesis and Carbon Metabolism Favor Arsenic Tolerance in Artemisia annua, a Medicinal Plant as Revealed by Homology-Based Proteomics
Figure 2
The 2-DE images of cytosolic protein extracts from Artemisia annua (a) control, (b) 100 μM, and (c) 150 μM As. All gels were run in triplicate. Proteins were extracted and separated by 2-DE and visualized by CBB staining. The protein (250 μg) was applied to pH 4–7 IPG dry strips with 12.5% linear vertical SDS-PAGE as the second dimension. The arrows with numbers on 2-DE gel indicate the differentially expressed proteins, which were further identified by MALDI-MS/MS analysis. The molecular mass marker (Sigma) and pI are indicated on the left side and above the gels, respectively.
