Enhanced Photosynthesis and Carbon Metabolism Favor Arsenic Tolerance in Artemisia annua, a Medicinal Plant as Revealed by Homology-Based Proteomics
Figure 4
Multivariate analysis (PCA analysis) of control, 100 and 150 μM As treatments using the ANOVA-based NIA array analysis tool. (a) Dendrogram showing hierarchical clustering of biological replicates of the control and As treatments. The expression clusters are numbered from 1 to 5. (b) Two-dimensional PCA biplots showing associations between experimental groups and protein spots generated by principal component analysis (PCA). Experimental groups (left) and protein spots (right) were plotted in the first two component spaces. Spots that are clustered together on the biplot should have similar expression profiles. (c) Protein spot abundance clustering based on PCA. For each PC, two clusters of proteins were identified that were positively and negatively correlated with the PC. Protein clustering was performed sequentially starting from the first PC. Proteins that were already clustered with a PC were not included in the clusters associated with subsequent PCs. The different colors of the spots represent the average expression patterns of proteins. Proteins are more expressed in those tissues which are located in the same area of the graph.
