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International Journal of Polymer Science
Volume 2010 (2010), Article ID 296094, 22 pages
http://dx.doi.org/10.1155/2010/296094
Review Article

Surface Engineered Polymeric Biomaterials with Improved Biocontact Properties

Laboratory for Advanced Materials, Department of Polymer Engineering, University of Chemical Technology and Metallurgy, 8 Kl. Ohridski Boulevard., 1756 Sofia, Bulgaria

Received 4 August 2009; Revised 24 November 2009; Accepted 31 March 2010

Academic Editor: Shanfeng Wang

Copyright © 2010 Todorka G. Vladkova. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. N. Minoura, S. Aiba, Y. Fujiwara, N. Koshizaki, and Y. Imai, “The interaction of cultured cells with membranes composed of random and block copolypeptides,” Journal of Biomedical Materials Research, vol. 23, no. 2, pp. 267–279, 1989. View at Scopus
  2. R. Large, MRS Bulletin, vol. 31, p. 447, 2004.
  3. B.-S. Kim and D. J. Mooney, “Development of biocompatible synthetic extracellular matrices for tissue engineering,” Trends in Biotechnology, vol. 16, no. 5, pp. 224–229, 1998. View at Publisher · View at Google Scholar · View at Scopus
  4. R. Langer and J. P. Vacanti, “Tissue engineering,” Science, vol. 260, no. 5110, pp. 920–926, 1993. View at Scopus
  5. L. L. Hench and J. M. Polak, “Third-generation biomedical materials,” Science, vol. 295, no. 5557, pp. 1014–1017, 2002. View at Scopus
  6. J. Anderson, “A forecast of the future for biomaterials,” in Proceedings of the Future of Biomedical Materials (key note), Symposium, Imperial Colleage London, September 2005.
  7. S. E. Sakiyama-Elbert and J. A. Hubbell, “Functional biomaterials: design of novel biomaterials,” Annual Review of Materials Science, vol. 31, pp. 183–201, 2001. View at Publisher · View at Google Scholar · View at Scopus
  8. L. Bachakova, Physiological Research, vol. 53, pp. 35–45, 2004.
  9. C.-G. Gölander, Preparation and properties of functionalized polymer surfaces, Ph.D. thesis, The Royal Institute of Technology, Stockholm, Sweden, 1986.
  10. M. Malmsten, Ed., Biopolymers at Interfaces, Marcel Dekker, New York, NY, USA, 1998.
  11. S. Pasche, Mechanisms of protein resistance of adsorbed PEG-Graft copolymers, D.Sc. thesis, Swiss Federal Institute of Technology, Zurich, Switzerland, 2004.
  12. S. Drotleff, Polymers and protein-conjugates for tissue engineering, Ph.D. thesis, University of Regensburg, Regensburg, Germany, 2006.
  13. V. Hlady, R. A. VanWagenen, and J. D. Andrade, in Surface and Interfacial Aspects of Biomedical Polymers, J. D. Andrade, Ed., vol. 2, p. 81, Plenum Press, New York, NY, USA, 1985.
  14. M. Jager, C. Zilkens, K. Zanger, and R. Krauspe, “Significance of nano- and microtopography for cell-suraface interactions in orthopedic implants,” Journal of Biomedicine and Biotechnology, vol. 2007, Article ID 69036, 2007. View at Publisher · View at Google Scholar · View at PubMed
  15. G. Altankov, Cell/biomaterial surfaces interaction, D.Sc. thesis, Institute of Biophysics, BAS, Sofia, Bulgaria, 2003.
  16. F. Grinnell and M. K. Feld, “Adsorption characteristics of plasma fibronectin in relationship to biological activity,” Journal of Biomedical Materials Research, vol. 15, no. 3, pp. 363–381, 1981.
  17. J. D. Andrade and V. Hlady, “Protein adsorption and materials biocompatibility. A tutorial review and suggested hypothesis,” Progress in Surface Science, vol. 79, pp. 1–63, 1986.
  18. V. Prasad, “Strategies for de novo engineering of tissues,” in Proceedings of the NATO ARW Nano-Engineered Systems for Regenerative Medicine, Varna, Bulgaria, September 2007.
  19. J. Panell, “Material surface effects on biological interactions,” in Proceedings of the NATO ARW Nano-Engineered Systems for Regenerative Medicine, Varna, Bulgaria, September 2007.
  20. J. Champion, “UROs, tacos, worms and surfboards: what they teach about material-cell interactions,” in Proceedings of the NATO ARW Nano-Engineered Systems for Regenerative Medicine, Varna, Bulgaria, September 2007.
  21. W. Senaratne, P. Sengupta, V. Jakubek, D. Holowka, C. K. Ober, and B. Baird, “Functionalized surface arrays for spatial targeting of immune cell signaling,” Journal of the American Chemical Society, vol. 128, no. 17, pp. 5594–5595, 2006. View at Publisher · View at Google Scholar · View at PubMed
  22. W. Senaratne, L. Andruzzi, and C. K. Ober, “Self-assembled monolayers and polymer brushes in biotechnology: current applications and future perspectives,” Biomacromolecules, vol. 6, no. 5, pp. 2427–2448, 2005. View at Publisher · View at Google Scholar · View at PubMed
  23. S. Sano, K. Kato, and Y. Ikada, “Introduction of functional groups onto the surface of polyethylene for protein immobilization,” Biomaterials, vol. 14, no. 11, pp. 817–822, 1993. View at Scopus
  24. A. Hoffman, Annals of the New York Academy of Sciences, pp. 97–101, 1988.
  25. P. K. Chu, J. Y. Chen, L. P. Wang, and N. Huang, “Plasma-surface modification of biomaterials,” Materials Science and Engineering, vol. 36, no. 5-6, pp. 143–206, 2002.
  26. C. M. Chan, Polymer Surface Modification and Characterization, chapters 5–7, Hanser, Brookfield, Wis, USA, 1993.
  27. F. Abbasi, H. Mirzadeh, and A.-A. Katbab, “Modification of polysiloxane polymers for biomedical applications: a review,” Polymer International, vol. 50, no. 12, pp. 1279–1287, 2001. View at Publisher · View at Google Scholar
  28. U. Vohrer, “Interfacial engineering of functional textiles for biomedical applications,” in Plasma Technologies for Textiles, R. Shishoo, Ed., p. 202, CRC Press; Boca Raton, Fla, USA, Woodhead, Cambridge, UK, 2007.
  29. C.-M. Chan, T.-M. Ko, and H. Hiraoka, “Polymer surface modification by plasmas and photons,” Surface Science Reports, vol. 24, no. 1-2, pp. 1–54, 1996.
  30. K. R. Rau, Surface modification of biomaterials by pulsed laser ablasion deposition and plasma/gamma polymerization, Ph.D. thesis, University of Florida, Gainesville, Fla, USA, 2001.
  31. R. Shishoo, Ed., Plasma Technologies for Textiles, CRC Press; Boca Raton, Fla, USA, Woodhead, Cambridge, UK, 2007.
  32. A. Denizli, E. Piskin, V. Dixit, M. Arthur, and G. Gitnick, “Collagen and fibronectin immobilization of PHEMA microcarriers for hepatocyte attachment,” International Journal of Artificial Organs, vol. 18, no. 2, pp. 90–95, 1995.
  33. S. Jaumotte-Thelen, I. Dozot-Dupont, J. Marchand-Brynaert, and Y.-J. Schneider, “Covalent grafting of fibronectin and asialofetuin at surface of poly(ethylene terephthalate) track-etched membranes improves adhesion but not differentiation of rat hepatocytes,” Journal of Biomedical Materials Research, vol. 32, no. 4, pp. 569–582, 1996.
  34. R. Chen and J. A. Hunt, “Biomimetic materials processing for tissue-engineering processes,” Journal of Materials Chemistry, vol. 17, no. 38, pp. 3974–3979, 2007. View at Publisher · View at Google Scholar
  35. S. P. Massia and J. A. Hubbell, “Human endothelial cell interactions with surface-coupled adhesion peptides on a nonadhesive glass substrate and two polymeric biomaterials,” Journal of Biomedical Materials Research, vol. 25, no. 2, pp. 223–242, 1991.
  36. Y. Ykada, M. Suzuki, and Y. Tamada, “Polymer surfaces possessing minimal interaction with blood components,” in Polymers as Biomaterials, Plenum Press, New York, NY, USA, 1984.
  37. S. E. Sakiyama-Elbert and J. A. Hubbell, “Functional biomaterials: design of novel biomaterials,” Annual Review of Materials Science, vol. 31, pp. 183–201, 2001. View at Publisher · View at Google Scholar
  38. B. D. Ratner, Biocompatibility of Clinical Implant Materials, vol. 2 of D. W. Williams, Eds., CRC Press, Boca Raton, Fla, USA, 1981.
  39. A. S. Hoffman, T. A. Horbett, and B. D. Ratner, “Interactions of blood and blood components at hydrogel interfaces,” Annals of the New York Academy of Sciences, vol. 283, pp. 372–382, 1977.
  40. W. Kim and J. Feijen, “Surface modification of polymers for improved blood compatibility,” CRC Critical Reviews in Biocompatibility, vol. 1, p. 229, 1985.
  41. K. Ishihara, Biocompatible Polymers, CRC Press, London, UK, 1994.
  42. B. D. Ratner and A. S. Hoffman, “Non-fouling surfaces,” in Biomaterials Science, B. D. Ratner, A. S. Hoffman, F. J. Schoen, and J. E. Lemons, Eds., pp. 197–201, Elsevier, San Diego, Calif, USA, 2nd edition, 2004.
  43. J. M. Harries, Ed., Poly(Ethylene Glycol) Chemistry. Biomedical and Biotechnical Applications, Plenum Press, New York, NY, USA, 1992.
  44. C.-G. Gölander, S. Jönsson, T. Vladkova, P. Stenius, and J. C. Eriksson, “Preparation and protein adsorption properties of photopolymerized hydrophilic films containing N-vinylpyrrolidone (NVP), acrylic acid (AA) or ethyleneoxide (EO) units as studied by ESCA,” Colloids & Surfaces, vol. 21, pp. 149–165, 1986.
  45. C.-G. Gölander, E.-S. Jönsson, T. Vladkova, et al., “Protein adsorption on some photo-polymerized hydrophilic films,” in Proceedings of the 8th International Symposium on Plasma Chemistry (IUPAC '87), vol. 8.27, Sofia, Bulgaria, July 1987.
  46. M. Malmsten and J. M. Van Alstine, “Adsorption of poly(ethylene glycol) amphiphiles to form coatings which inhibit protein adsorption,” Journal of Colloid and Interface Science, vol. 177, no. 2, pp. 502–512, 1996. View at Publisher · View at Google Scholar
  47. C.-G. Gölander, E.-S. Jönsson, and T. G. Vladkova, “EP022966 (B1); WO8602087 (A1); US4840851 (A); SU1729284 (A3); SE8404866 (L); SE444950 (B); NO861998 (A); JP62500307 (T); DK248986 (A); AU4965185 (A)”.
  48. T. Vladkova, “Modification of polymer surfaces for medical application,” in Proceedings of the 13th Science Conference “Modification of Polymers”, Kudowa Zdroj, Poland, September 1995.
  49. T. G. Vladkova, C.-G. Gölander, S. C. Christoskova, and E.-S. Jönsson, “Mechanically stable hydrophilic films based on oxialkylated macromers polymerizable by UV irradiation,” Polymers for Advanced Technologies, vol. 8, no. 6, pp. 347–350, 1997.
  50. T. Vladkova, Some Possibilities to Polymer Surface Modification, UCTM Ed. Centre, Sofia, Bulgaria, 2001.
  51. T. Vladkova, E.-S. Jönsson, and C.-G. Gölander, “Surface modification of natural rubber latex films by peg hydrogel coating,” Journal of University of Chemical Technology and Metallurgy, vol. 38, p. 131, 2003.
  52. T. Vladkova, “Surface modification of silicone rubber with poly(ethylene glycol) hydrogel coatings,” Journal of Applied Polymer Science, vol. 92, no. 3, pp. 1486–1492, 2004. View at Publisher · View at Google Scholar
  53. R. Bischoff and G. Bischoff, in Proceedings of the 11th Conference of the European Society of Biomechanics, Touluse, France, July 1998.
  54. B. P. Lee, K. Huang, F. N. Nunalee, K. Shull, and P. B. Messersmith, “Synthesis of 3,4-dihydroxyphenylalanine (DOPA) containing monomers and their co-polymerization with PEG-diacrylate to form hydrogels,” Journal of Biomaterials Science, Polymer Edition, vol. 15, no. 4, pp. 449–464, 2004. View at Publisher · View at Google Scholar
  55. I. K. Kwon and T. Matsuda, “Photo-iniferter-based thermoresponsive block copolymers composed of poly(ethylene glycol) and poly(N-isopropylacrylamide) and chondrocyte immobilization,” Biomaterials, vol. 27, no. 7, pp. 986–995, 2006. View at Publisher · View at Google Scholar · View at PubMed
  56. M. S. Hahn, L. J. Taite, J. J. Moon, M. C. Rowland, K. A. Ruffino, and J. L. West, “Photolithographic patterning of polyethylene glycol hydrogels,” Biomaterials, vol. 27, no. 12, pp. 2519–2524, 2006. View at Publisher · View at Google Scholar · View at PubMed
  57. S. Kizilel, E. Sawardecker, F. Teymour, and V. H. Pérez-Luna, “Sequential formation of covalently bonded hydrogel multilayers through surface initiated photopolymerization,” Biomaterials, vol. 27, no. 8, pp. 1209–1215, 2006. View at Publisher · View at Google Scholar · View at PubMed
  58. Y. Ito, H. Hasuda, M. Sakuragi, and S. Tsuzuki, “Surface modification of plastic, glass and titanium by photoimmobilization of polyethylene glycol for antibiofouling,” Acta Biomaterialia, vol. 3, no. 6, pp. 1024–1032, 2007. View at Publisher · View at Google Scholar · View at PubMed
  59. E. Kiss, C.-G. Gölander, and J. C. Eriksson, “Surface grafting of polyethyleneoxide optimized by means of ESCA,” Progress in Colloid & Polymer Science, vol. 74, no. 1, pp. 113–119, 1987. View at Publisher · View at Google Scholar
  60. C. L. Feng, Z. Zhang, R. Förch, W. Knoll, G. J. Vancso, and H. Schönherr, “Reactive thin polymer films as platforms for the immobilization of biomolecules,” Biomacromolecules, vol. 6, no. 6, pp. 3243–3251, 2005. View at Publisher · View at Google Scholar · View at PubMed
  61. R. Schlapak, P. Pammer, and P. Pammer, “Glass surfaces grafted with high-density poly(ethylene glycol) as substrates for DNA oligonucleotide microarrays,” Langmuir, vol. 22, no. 1, pp. 277–285, 2006. View at Publisher · View at Google Scholar · View at PubMed
  62. S. Patel, R. G. Thakar, J. Wong, S. D. McLeod, and S. Li, “Control of cell adhesion on poly(methyl methacrylate),” Biomaterials, vol. 27, no. 14, pp. 2890–2897, 2006. View at Publisher · View at Google Scholar · View at PubMed
  63. Y. Li, C. G. Worley, R. W. Linton, J. M. DeSimone, and E. T. Samulski, “Grafting of poly(ethylene oxide) to crosslinked polystyrene and polypropylene substrates with well-controlled chain length and functionalities,” Division of Polymer Chemistry—American Chemical Society, vol. 37, no. 2, pp. 737–738, 1996.
  64. K.C. Popat, G. Mor, C. A. Grimes, and T. A. Desai, “Surface modification of nanoporous alumina surfaces with poly(ethylene glycol),” Langmuir, vol. 20, no. 19, pp. 8035–8041, 2004. View at Publisher · View at Google Scholar · View at PubMed
  65. J. Piehler, A. Brecht, R. Valiokas, B. Liedberg, and G. Gauglitz, “A high-density poly(ethylene glycol) polymer brush for immobilization on glass-type surfaces,” Biosensors and Bioelectronics, vol. 15, no. 9-10, pp. 473–481, 2000. View at Publisher · View at Google Scholar
  66. Z.-K. Xu, F.-Q. Nie, C. Qu, L.-S. Wan, J. Wu, and K. Yao, “Tethering poly(ethylene glycol)s to improve the surface biocompatibility of poly(acrylonitrile-co-maleic acid) asymmetric membranes,” Biomaterials, vol. 26, no. 6, pp. 589–598, 2005. View at Publisher · View at Google Scholar · View at PubMed
  67. J. Groll, T. Ameringer, J. P. Spatz, and M. Moeller, “Ultrathin coatings from isocyanate-terminated star PEG prepolymers: layer formation and characterization,” Langmuir, vol. 21, no. 5, pp. 1991–1999, 2005. View at Publisher · View at Google Scholar · View at PubMed
  68. I. Choi, S. K. Kang, J. Lee, Y. Kim, and J. Yi, “In situ observation of biomolecules patterned on a PEG-modified Si surface by scanning probe lithography,” Biomaterials, vol. 27, no. 26, pp. 4655–4660, 2006. View at Publisher · View at Google Scholar · View at PubMed
  69. H. Xu, J. L. Kaar, A. J. Russell, and W. R. Wagner, “Characterizing the modification of surface proteins with poly(ethylene glycol) to interrupt platelet adhesion,” Biomaterials, vol. 27, no. 16, pp. 3125–3135, 2006. View at Publisher · View at Google Scholar · View at PubMed
  70. Y. G. Ko, Y. H. Kim, K. D. Park, et al., “Immobilization of poly(ethylene glycol) or its sulfonate onto polymer surfaces by ozone oxidation,” Biomaterials, vol. 22, no. 15, pp. 2115–2123, 2001. View at Publisher · View at Google Scholar
  71. R. P. Sebra, K. S. Masters, C. Y. Cheung, C. N. Bowman, and K. S. Anseth, “Detection of antigens in biologically complex fluids with photografted whole antibodies,” Analytical Chemistry, vol. 78, no. 9, pp. 3144–3151, 2006. View at Publisher · View at Google Scholar · View at PubMed
  72. M. Beyer, T. Felgenhauer, F. R. Bischoff, F. Breitling, and V. Stadler, “A novel glass slide-based peptide array support with high functionality resisting non-specific protein adsorption,” Biomaterials, vol. 27, no. 18, pp. 3505–3514, 2006. View at Publisher · View at Google Scholar · View at PubMed
  73. D. Xiao, H. Zhang, and M. Wirth, “Chemical modification of the surface of poly(dimethylsiloxane) by atom-transfer radical polymerization of acrylamide,” Langmuir, vol. 18, no. 25, pp. 9971–9976, 2002. View at Publisher · View at Google Scholar
  74. T. Goda, T. Konno, M. Takai, T. Moro, and K. Ishihara, “Biomimetic phosphorylcholine polymer grafting from polydimethylsiloxane surface using photo-induced polymerization,” Biomaterials, vol. 27, no. 30, pp. 5151–5160, 2006. View at Publisher · View at Google Scholar · View at PubMed
  75. T. Vladkova, N. Krasteva, A. Kostadinova, and G. Altankov, “Preparation of PEG-coated surfaces and a study for their interaction with living cells,” Journal of Biomaterials Science, Polymer Edition, vol. 10, no. 6, pp. 609–620, 1999.
  76. S. P. Massia, J. Stark, and D. S. Letbetter, “Surface-immobilized dextran limits cell adhesion and spreading,” Biomaterials, vol. 21, no. 22, pp. 2253–2261, 2000. View at Publisher · View at Google Scholar
  77. S. K. Thanawala and M. K. Chaudhury, “Surface modification of silicone elastomer using perfluorinated ether,” Langmuir, vol. 16, no. 3, pp. 1256–1260, 2000. View at Publisher · View at Google Scholar
  78. S. Yang, J. Wang, K. Ogino, S. Valiyaveettil, and C. K. Ober, “Low-surface-energy fluoromethacrylate block copolymers with patternable elements,” Chemistry of Materials, vol. 12, no. 1, pp. 33–40, 2000. View at Publisher · View at Google Scholar
  79. L. Andruzzi, W. Senaratne, A. Hexemer, et al., “Oligo(ethylene glycol) containing polymer brushes as bioselective surfaces,” Langmuir, vol. 21, no. 6, pp. 2495–2504, 2005. View at Publisher · View at Google Scholar · View at PubMed
  80. S. S. Hwang, C. K. Ober, S. Perutz, D. R. Iyengar, L. A. Schneggenburger, and E. J. Kramer, “Block copolymers with low surface energy segments: siloxane- and perfluoroalkane-modified blocks,” Polymer, vol. 36, no. 6, pp. 1321–1325, 1995.
  81. S. Krishnan, R. Ayothi, A. Hexemer, et al., “Anti-biofouling properties of comblike block copolymers with amphiphilic side chains,” Langmuir, vol. 22, no. 11, pp. 5075–5086, 2006. View at Publisher · View at Google Scholar · View at PubMed
  82. S. Krishnan, N. Wang, C. Ober, C. Finlay, J. Callow, and M. Callow, Biomacromolecules, vol. 117, pp. 2775–2783, 2009.
  83. T. Vladkova, “Modification of polymer surfaces for medical application,” in Proceedings of the 13th International Science Conference “Modification of Polymers“, Kudowa Zdroj, Poland, September 1995.
  84. Y. Kicheva, T. Vladkova, V. Kostov, and C.-G. Gölander, “Preparation of surface modified PVC drain tubing and in vivo study of their biocompatibility,” Journal of the University of Chemical Technology and Metallurgy, vol. 37, pp. 77–84, 2002.
  85. Y. Kicheva, V. Kostov, M. Mateev, and T. Vladkova, “In vitro and in vivo evaluation of biocompatibility of PVC materials with modified surfaces,” in Proceedings of the 6th Colloquium on Biomaterials, Aahen, Germany, September 2002.
  86. P. Sioshansi and E. J. Tobin, “Surface treatment of biomaterials by ion beam processes,” Surface and Coatings Technology, vol. 83, no. 1–3, pp. 175–182, 1996. View at Publisher · View at Google Scholar
  87. M. Szicher, P. Sioshansi, and E. Frish, Biomaterials for the 1990s: Polyurethanes, Silicones, and Ion Beam Modification Techniques, Part 2, Spire, Bedford, Mass, USA, 1990.
  88. M. Inoue, Y. Suzuki, and T. Takagi, “Review of Ion Engineering Center and related projects in Ion Engineering Research Institute,” Nuclear Instruments and Methods in Physics Research B, vol. 121, no. 1–4, pp. 1–6, 1997.
  89. I. F. Husein, C. Chan, and P. K. Chu, “Chemical structure modification of silicone surfaces by plasma immersion ion implantation,” Journal of Materials Science Letters, vol. 19, no. 21, pp. 1883–1885, 2000. View at Publisher · View at Google Scholar
  90. I. F. Husein, C. Chan, S. Qin, and P. K. Chu, “The effect of high-dose nitrogen plasma immersion ion implantation on silicone surfaces,” Journal of Physics D, vol. 33, no. 22, pp. 2869–2874, 2000.
  91. C. Satriano, S. Carnazza, S. Guglielmino, and G. Marletta, “Differential cultured fibroblast behavior on plasma and ion-beam-modified polysiloxane surfaces,” Langmuir, vol. 18, no. 24, pp. 9469–9475, 2002. View at Publisher · View at Google Scholar
  92. B. Bhushan, D. Hansford, and K. K. Lee, “Surface modification of silicon and polydimethylsiloxane surfaces with vapor-phase-deposited ultrathin fluorosilane films for biomedical nanodevices,” Journal of Vacuum Science and Technology A, vol. 24, no. 4, pp. 1197–1202, 2006. View at Publisher · View at Google Scholar
  93. W. J. Sear, P. P. Capek, and F. J. Clubb, in Proceedings of the 40th Annual Conference on ADAIO, New York, NY, USA, April 1998.
  94. L. Hakelius and L. Ohlsen, “A clinical comparison of the tendency to capsular contracture between smooth and textured gel-filled silicone mammary implants,” Plastic and Reconstructive Surgery, vol. 90, no. 2, pp. 247–254, 1992.
  95. K. Ishihara, “Blood compatible polymers,” in Biomedical Applications of Polymeric Materials, T. Tsuruta, T. Hyashi, K. Kataoka, K. Ishihara, and Y. Kimura, Eds., CRC Press, Boca Raton, Fla, USA, 1993.
  96. D.-C. Sin, H.-L. Kei, and X. Miao, “Surface coatings for ventricular assist devices,” Expert Review of Medical Devices, vol. 6, no. 1, pp. 51–60, 2009. View at Publisher · View at Google Scholar · View at PubMed
  97. P. Claesson and C.-G. Gölander, Colloids and Surfaces, vol. 20, p. 186, 1995.
  98. D. L. Coleman, D. E. Gregonis, and J. D. Andrade, “Blood-materials interactions: the minimum interfacial free energy and the optimum polar/apolar ratio hypotheses,” Journal of Biomedical Materials Research, vol. 16, no. 4, pp. 381–398, 1982.
  99. M. D. Lelah, J. A. Pierce, L. K. Lambrecht, and S. L. Cooper, “Polyether-urethane ionomers: surface property/ex vivo blood compatibility relationships,” Journal of Colloid and Interface Science, vol. 104, no. 2, pp. 422–439, 1985.
  100. S. Nagaoka, Y. Mory, and S. Nishiumi, “Interaction between blood components and hydrogels with polyoxyethylene chains,” Polymer Preprints, vol. 34, no. 1, p. 67, 1983.
  101. H. Tanzawa, “Biomedical polymers: current status and overview,” in Biomedical Applications of Polymeric Materials, T. Tsuruta, T. Hayashi, K. Kataoka, K. Ishihara, and Y. Kimura, Eds., p. 12, CRC Press, Boca Raton, Fla, USA, 1993.
  102. S. Hattori, J. D. Andrade, J. B. Hibbs Jr., D. E. Gregonis, and R. N. King, “Fibroblast cell proliferation on charged hydroxyethyl methacrylate copolymers,” Journal of Colloid and Interface Science, vol. 104, no. 1, pp. 72–78, 1985.
  103. N. Minoura, S. Aiba, Y. Fujiwara, N. Koshizaki, and Y. Imai, “The interaction of cultured cells with membranes composed of random and block copolypeptides,” Journal of Biomedical Materials Research, vol. 23, no. 2, pp. 267–279, 1989.
  104. H. Sato, A. Nakajima, T. Hayashi, G. W. Chen, and Y. Noishiki, “Microheterophase structure, permeability, and biocompatibility of A-B-A triblock copolymer membranes composed of poly(gamma-ethyl L-glutamate) as the A component and polybutadiene as the B component,” Journal of Biomedical Materials Research, vol. 19, no. 9, pp. 1135–1155, 1985.
  105. T. Okano, T. Aoyagi, K. Kataoka, et al., “Hydrophilic-hydrophobic microdomain surfaces having an ability to suppress platelet aggregation and their in vitro antithrombogenicity,” Journal of Biomedical Materials Research, vol. 20, no. 7, pp. 919–927, 1986.
  106. T. Okano, M. Uruno, N. Sugiyama, et al., “Suppression of platelet activity on microdomain surfaces of 2-hydroxyethyl methacrylate-polyether block copolymers,” Journal of Biomedical Materials Research, vol. 20, no. 7, pp. 1035–1047, 1986.
  107. K. D. Park, T. Okano, C. Nojiri, and S. W. Kim, “Heparin immobilization onto segmented polyurethaneurea surfaces—effect of hydrophilic spacers,” Journal of Biomedical Materials Research, vol. 22, no. 11, pp. 977–992, 1988.
  108. T. Okada and Y. Ikada, Makromolekulare Chemie, vol. 192, p. 1705, 1991.
  109. S. M. Kirkham and M. E. Dangel, “The keratoprosthesis: improved biocompatability through design and surface modification,” Ophthalmic Surgery, vol. 22, no. 8, pp. 455–461, 1991.
  110. T. Okada and Y. Ikada, “Tissue reactions to subcutaneously implanted, surface-modified silicones,” Journal of Biomedical Materials Research, vol. 27, no. 12, pp. 1509–1518, 1993. View at Publisher · View at Google Scholar · View at PubMed
  111. Y. Kinoshita, T. Kuzuhara, M. Kirigakubo, M. Kobayashi, K. Shimura, and Y. Ikada, “Soft tissue reaction to collagen-immobilized porous polyethylene: subcutaneous implantation in rats for 20 wk,” Biomaterials, vol. 14, no. 3, pp. 209–215, 1993. View at Publisher · View at Google Scholar
  112. H. Mirzadeh, A. A. Katbab, and R. P. Burford, “CO2-pulsed laser induced surface grafting of acrylamide onto ethylene-propylene-rubber (EPR)—I,” Radiation Physics and Chemistry, vol. 41, no. 3, pp. 507–519, 1993.
  113. H. Mirzadeh, M. T. Khorasani, A. A. Katbab, et al., “Biocompatibility evaluation of laser-induced AAm and HEMA grafted EPR—part 1: in-vitro study,” Clinical Materials, vol. 16, no. 4, pp. 177–187, 1994. View at Publisher · View at Google Scholar
  114. H. Mirzadeh, A. A. Katbab, M. T. Khorasani, R. P. Burford, E. Gorgin, and A. Golestani, “Cell attachment to laser-induced AAm- and HEMA-grafted ethylene-propylene rubber as biomaterial: in vivo study,” Biomaterials, vol. 16, no. 8, pp. 641–648, 1995. View at Publisher · View at Google Scholar
  115. M. T. Khorasani, H. Mirzadeh, and P. G. Sammes, “Laser induced surface modification of polydimethylsiloxane as a super-hydrophobic material,” Radiation Physics and Chemistry, vol. 47, no. 6, pp. 881–888, 1996. View at Publisher · View at Google Scholar
  116. H. Mirzadeh, M. Khorasani, and P. G. Sammes, “Laser surface modification of polymers. A novel technique for the preparation of blood compatible materials (II): in vitro assay,” Iranian Polymer Journal, vol. 7, no. 1, pp. 5–13, 1998.
  117. M. T. Korasani, H. Mirzadeh, and P. G. Sammes, in Surface Modification Technologies, T. Sudarshan, A. Khor, and M. Eandin, Eds., p. 499, Institute of Materials, London, UK, 1996.
  118. M. T. Khorasani, H. Mirzadeh, and P. G. Sammes, “Laser surface modification of polymers to improve biocompatibility: HEMA grafted PDMS, in vitro assay—III,” Radiation Physics and Chemistry, vol. 55, no. 5-6, pp. 685–689, 1999. View at Publisher · View at Google Scholar
  119. R. Lasson, Chemical constitution and biological properties of heparinized surface, Ph.D. thesis, Karolinska Institute, Stockholm, Sweden, 1980.
  120. H. J. Steffen, J. Schmidt, and A. Gonzalez-Elipe, “Biocompatible surfaces by immobilization of heparin on diamond-like carbon films deposited on various substrates,” Surface and Interface Analysis, vol. 29, no. 6, pp. 386–391, 2000. View at Publisher · View at Google Scholar
  121. Y. Susuki, M. Kasakabe, M. Iwaki, and M. Suzuki, Nuclear Instruments and Methods in Physics Research, vol. B32, p. 120, 1998.
  122. Y. Susuki, C. Swapp, M. Kasakabe, and M. Iwaki, Nuclear Instruments and Methods in Physics Research, vol. B46, p. 354, 1990.
  123. Y. Suzuki, M. Kusakabe, M. Iwaki, H. Akiba, K. Kusakabe, and S. Sato, “In vivo evaluation of antithrombogenicity for ion implanted silicone usineg in -111-tropolone-platelets,” Japanese Journal of Artificial Organs, vol. 19, no. 3, pp. 1092–1095, 1990.
  124. P. R. Udall, in Dialysis Therapy, A. R. Nilsen and R. N. Fine, Eds., Hanley & Belfus, Philadelphia, Pa, USA, 2nd edition, 1993.
  125. J. Anderson, “The future of biomedical materials (key note),” in Proceedings of a Forecast of the Future for Biomaterials, Imperial Colleage, September 2005.
  126. A. R. Boccaccini and J. J. Blaker, “Bioactive composite materials for tissue engineering scaffolds,” Expert Review of Medical Devices, vol. 2, no. 3, pp. 303–317, 2005. View at Publisher · View at Google Scholar · View at PubMed
  127. E. Eisenbarth, “Biomaterials for tissue engineering,” Advanced Engineering Materials, vol. 9, no. 12, pp. 1051–1060, 2007. View at Publisher · View at Google Scholar
  128. M. M. Stevens, R. P. Marini, D. Schaefer, J. Aronson, R. Langer, and V. P. Shastri, “In vivo engineering of organs: the bone bioreactor,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 32, pp. 11450–11455, 2005. View at Publisher · View at Google Scholar · View at PubMed
  129. C. Weinand, I. Pomerantseva, C. M. Neville, et al., “Hydrogel-β-TCP scaffolds and stem cells for tissue engineering bone,” Bone, vol. 38, no. 4, pp. 555–563, 2006. View at Publisher · View at Google Scholar · View at PubMed
  130. J. Jones, Materials Today, vol. 9, no. 12, p. 35, 2006.
  131. H. J. Chung and T. G. Park, “Surface engineered and drug releasing pre-fabricated scaffolds for tissue engineering,” Advanced Drug Delivery Reviews, vol. 59, no. 4-5, pp. 249–262, 2007. View at Publisher · View at Google Scholar · View at PubMed
  132. G. Altankov, “Development of provisional extracellular matrix on the biomaterial interface: lessons from in vitro cell culture,” in Proceedings of the NATO Advanced Research Workshop on Nanoengineered Systems for Regenerative Medicine, Varna, Bulgaria, September 2007.
  133. M. J. Lydon, T. W. Minett, and B. J. Tighe, “Cellular interactions with synthetic polymer surfaces in culture,” Biomaterials, vol. 6, no. 6, pp. 396–402, 1985.
  134. K. Smetana Jr., “Cell biology of hydrogels,” Biomaterials, vol. 14, no. 14, pp. 1046–1050, 1993. View at Publisher · View at Google Scholar
  135. H.-B. Lin, W. Sun, D. F. Mosher, et al., “Synthesis, surface, and cell-adhesion properties of polyurethanes containing covalently grafted RGD-peptides,” Journal of Biomedical Materials Research, vol. 28, no. 3, pp. 329–342, 1994.
  136. R. G. Flemming, C. J. Murphy, G. A. Abrams, S. L. Goodman, and P. F. Nealey, “Effects of synthetic micro- and nano-structured surfaces on cell behavior,” Biomaterials, vol. 20, no. 6, pp. 573–588, 1999. View at Publisher · View at Google Scholar
  137. K. Saha, J. F. Pollock, D. V. Schaffer, and K. E. Healy, “Designing synthetic materials to control stem cell phenotype,” Current Opinion in Chemical Biology, vol. 11, no. 4, pp. 381–387, 2007. View at Publisher · View at Google Scholar · View at PubMed
  138. L. Moroni, J. A. A. Hendriks, R. Schotel, J. R. de Wijn, and C. A. van Blitterswijk, “Design of biphasic polymeric 3-dimensional fiber deposited scaffolds for cartilage tissue engineering applications,” Tissue Engineering, vol. 13, no. 2, pp. 361–371, 2007. View at Publisher · View at Google Scholar · View at PubMed
  139. D.-A. Wang, S. Varghese, B. Sharma, et al., “Multifunctional chondroitin sulphate for cartilage tissue-biomaterial integration,” Nature Materials, vol. 6, no. 5, pp. 385–392, 2007. View at Publisher · View at Google Scholar · View at PubMed
  140. H. Lee, S. M. Dellatore, W. M. Miller, and P. B. Messersmith, “Mussel-inspired surface chemistry for multifunctional coatings,” Science, vol. 318, no. 5849, pp. 426–430, 2007. View at Publisher · View at Google Scholar · View at PubMed
  141. D. S. W. Benoit, A. R. Durney, and K. S. Anseth, “The effect of heparin-functionalized PEG hydrogels on three-dimensional human mesenchymal stem cell osteogenic differentiation,” Biomaterials, vol. 28, no. 1, pp. 66–77, 2007. View at Publisher · View at Google Scholar · View at PubMed
  142. M. P. Ludolf and J. A. Hubbel, “Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering,” Nature Biotechnology, vol. 25, pp. 47–55, 2007.
  143. H. J. Chung and T. G. Park, “Surface engineered and drug releasing pre-fabricated scaffolds for tissue engineering,” Advanced Drug Delivery Reviews, vol. 59, no. 4-5, pp. 249–262, 2007. View at Publisher · View at Google Scholar · View at PubMed
  144. G. Altankov, T. Vladkova, N. Krasteva, A. Kostadinova, and I. Keranov, “Preparation of protein repellent PEI/PEG coatings and fibronectin reorganization study on the coated surfaces,” Journal of the University of Chemical Technology and Metallurgy, vol. 44, no. 4, pp. 333–340, 2009.
  145. G. Altankov and T. Groth, “Reorganization of substratum-bound fibronectin on hydrophilic and hydrophobic materials is related to biocompatibility,” Journal of Materials Science, vol. 5, no. 9-10, pp. 732–737, 1994. View at Publisher · View at Google Scholar
  146. G. Altankov and T. Groth, “Fibronectin matrix formation by human fibroblasts on surfaces varying in wettability,” Journal of Biomaterials Science, Polymer Edition, vol. 8, no. 4, pp. 299–310, 1996.
  147. C. F. Amstein and P. A. Hartman, “Adaptation of plastic surfaces for tissue culture by glow discharge,” Journal of Clinical Microbiology, vol. 2, no. 1, pp. 46–54, 1975.
  148. G. N. Hannan and B. R. McAuslan, “Immobilized serotonin: a novel substrate for cell culture,” Experimental Cell Research, vol. 171, no. 1, pp. 153–163, 1987.
  149. J. A. Chinn, T. A. Horbett, B. D. Ratner, M. B. Schway, Y. Haque, and S. D. Hauschka, “Enhancement of serum fibronectin adsorption and the clonal plating efficiencies of Swiss mouse 3T3 fibroblast and MM14 mouse myoblast cells on polymer substrates modified by radiofrequency plasma deposition,” Journal of Colloid and Interface Science, vol. 127, no. 1, pp. 67–87, 1989.
  150. S.-D. Lee, G.-H. Hsiue, and C.-Y. Kao, “Preparation and characterization of a homobifunctional silicone rubber membrane grafted with acrylic acid via plasma-induced graft copolymerization,” Journal of Polymer Science A, vol. 34, no. 1, pp. 141–148, 1996.
  151. C. Satriano, E. Conte, and G. Marletta, “Surface chemical structure and cell adhesion onto ion beam modified polysiloxane,” Langmuir, vol. 17, no. 7, pp. 2243–2250, 2001. View at Publisher · View at Google Scholar
  152. C. Satriano, S. Carnazza, S. Guglielmino, and G. Marletta, “Differential cultured fibroblast behavior on plasma and ion-beam-modified polysiloxane surfaces,” Langmuir, vol. 18, no. 24, pp. 9469–9475, 2002. View at Publisher · View at Google Scholar
  153. T. Vladkova, I. Keranov, and G. Altankov, “Preparation and properties of PDMS surfaces grafted with acrylic acid via plasma pretment or ion-beam induced graft co-polymerization,” in Proceedings of the 4th International Conference on Chemical Societies of the South-Eastern European Countries (ICOSECS '04), Belgrad, Serbia, July 2004, A-P 26.
  154. T. Vladkova, I. Keranov, P. Dineff, and G. Altankov, “Ion-beam assisted surface modification of PDMS,” in Proceedings of the 18th Congres of Chemists and Technologiest of Macedonia, Ohrid, Macedonia, September 2004, PPM-16.
  155. R. Hippler, S. Pfau, M. Schmidt, and K. Schönbach, Eds., Low Temperature Plasma Physics. Fundamental Aspects and Applications, Willey-VCH, Berlin, Germany, 2000.
  156. T. G. Vladkova, I. L. Keranov, P. D. Dineff, et al., “Plasma based Ar+ beam assisted poly(dimethylsiloxane) surface modification,” Nuclear Instruments and Methods in Physics Research B, vol. 236, no. 1–4, pp. 552–562, 2005. View at Publisher · View at Google Scholar
  157. I. Keranov, T. G. Vladkova, M. Minchev, A. Kostadinova, G. Altankov, and P. Dineff, “Topography characterization and initial cellular interaction of plasma-based ar1 beam-treated PDMS surfaces,” Journal of Applied Polymer Science, vol. 111, no. 5, pp. 2637–2646, 2009. View at Publisher · View at Google Scholar
  158. J. M. Oliveira, I. B. Leonor, and R. L. Reis, “Preparation of bioactive coatings on the surface of bioinert polymers through an innovative auto-catalytic electroless route,” Key Engineering Materials, vol. 284–286, pp. 203–206, 2005.
  159. T. Kawai, C. Ohtsuki, M. Kamitakahara, et al., “A comparative study of apatite deposition on polyamide films containing different functional groups under a biomimetic condition,” Journal of the Ceramic Society of Japan, vol. 113, no. 1321, pp. 588–592, 2005. View at Publisher · View at Google Scholar
  160. M. Morra, “Biomolecular modification of implant surfaces,” Expert Review of Medical Devices, vol. 4, no. 3, pp. 361–372, 2007. View at Publisher · View at Google Scholar · View at PubMed
  161. J. L. West, “Biofunctional polymers,” in Encyclopedia of Biomaterials and Biomedical Engineering, pp. 89–95, 2007. View at Publisher · View at Google Scholar
  162. A. Denizli, E. Piskin, V. Dixit, M. Arthur, and G. Gitnick, “Collagen and fibronectin immobilization of PHEMA microcarriers for hepatocyte attachment,” International Journal of Artificial Organs, vol. 18, no. 2, pp. 90–95, 1995.
  163. S. Jaumotte-Thelen, I. Dozot-Dupont, J. Marchand-Brynaert, and Y.-J. Schneider, “Covalent grafting of fibronectin and asialofetuin at surface of poly(ethylene terephthalate) track-etched membranes improves adhesion but not differentiation of rat hepatocytes,” Journal of Biomedical Materials Research, vol. 32, no. 4, pp. 569–582, 1996.
  164. R. Chen and J. A. Hunt, “Biomimetic materials processing for tissue-engineering processes,” Journal of Materials Chemistry, vol. 17, no. 38, pp. 3974–3979, 2007. View at Publisher · View at Google Scholar
  165. B. B. Hole, J. A. Schwarz, J. L. Gilbert, and B. L. Atkinson, “A study of biologically active peptide sequences (P-15) on the surface of an ABM scaffold (PepGen P-15) using AFM and FTIR,” Journal of Biomedical Materials Research A, vol. 74, no. 4, pp. 712–721, 2005. View at Publisher · View at Google Scholar · View at PubMed
  166. C. T. Laurencin and L. S. Nain, Eds., Nanotechnology and Tissue Engineering: The Scaffold, CRC Press, Boca Raton, Fla, USA, 2009.
  167. C. R. Nuttelman, D. J. Mortisen, S. M. Henry, and K. S. Anseth, “Attachment of fibronectin to poly(vinyl alcohol) hydrogels promotes NIH3T3 cell adhesion, proliferation, and migration,” Journal of Biomedical Materials Research, vol. 57, no. 2, pp. 217–223, 2001.
  168. R. S. Braty, J. Crean, D. Lappin, and C. Godson, Journal of Biomedical Materials Research, vol. 56, pp. 78–82, 2001.
  169. M. P. Lutolf and J. A. Hubbell, “Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering,” Nature Biotechnology, vol. 23, no. 1, pp. 47–55, 2005. View at Publisher · View at Google Scholar · View at PubMed
  170. U. Hersel, C. Dahmen, and H. Kessler, “RGD modified polymers: biomaterials for stimulated cell adhesion and beyond,” Biomaterials, vol. 24, no. 24, pp. 4385–4415, 2003. View at Publisher · View at Google Scholar
  171. A. Karkhaneh, H. Mirzadeh, and A. R. Ghaffariyeh, “Two-step plasma surface modification of PDMS with mixture of HEMA and AAC: collagen immobilization and in vitro assays,” in Proceedings of the 5th IASTED International Conference on Biomedical Engineering, pp. 433–438, Innsbruck, Austria, February 2007.
  172. V. B. Ivanov, J. Behnisch, A. Hollander, F. Mehdorn, and H. Zimmermann, “Determination of functional groups on polymer surfaces using fluorescence labelling,” Surface and Interface Analysis, vol. 24, no. 4, pp. 257–262, 1996.
  173. J. Davies, C. S. Nunnerley, A. C. Brisley, et al., “Argon plasma treatment of polystyrene microtiter wells. Chemical and physical characterisation by contact angle, ToF-SIMS, XPS and STM,” Colloids and Surfaces A, vol. 174, no. 3, pp. 287–295, 2000. View at Publisher · View at Google Scholar
  174. F. Poncin-Epaillard and G. Legeay, “Surface engineering of biomaterials with plasma techniques,” Journal of Biomaterials Science, Polymer Edition, vol. 14, no. 10, pp. 1005–1028, 2003. View at Publisher · View at Google Scholar
  175. U. König, M. Nitschke, M. Pilz, F. Simon, C. Arnhold, and C. Werner, “Stability and ageing of plasma treated poly(tetrafluoroethylene) surfaces,” Colloids and Surfaces B, vol. 25, no. 4, pp. 313–324, 2002. View at Publisher · View at Google Scholar
  176. J. Johns, Materials Today, vol. 11, no. 5, p. 29, 2008.
  177. D. B. Haddow, D. A. Steele, R. D. Short, R. A. Dawson, and S. MacNeil, “Plasma-polymerized surfaces for culture of human keratinocytes and transfer of cells to an in vitro wound-bed model,” Journal of Biomedical Materials Research A, vol. 64, no. 1, pp. 80–87, 2003.
  178. B. Li, J. Chen, and J. H.-C. Wang, “RGD peptide-conjugated poly(dimethylsiloxane) promotes adhesion, proliferation, and collagen secretion of human fibroblasts,” Journal of Biomedical Materials Research A, vol. 79, no. 4, pp. 989–998, 2006. View at Publisher · View at Google Scholar · View at PubMed
  179. G. Sui, J. Wang, C.-C. Lee, et al., “Solution-phase surface modification in intact poly(dimethylsiloxane) microfluidic channels,” Analytical Chemistry, vol. 78, no. 15, pp. 5543–5551, 2006. View at Publisher · View at Google Scholar · View at PubMed
  180. Ch. Baquey, F. Palumbo, M. C. Porte-Durrieu, G. Legeay, A. Tressaud, and R. D'Agostino, “Plasma treatment of expanded PTFE offers a way to a biofunctionalization of its surface,” Nuclear Instruments and Methods in Physics Research B, vol. 151, no. 1–4, pp. 255–262, 1999. View at Publisher · View at Google Scholar
  181. S. Sano and S. Wong, Chemistry of Protein Conjugation and Cross-Linking, CRC Press, Boca Raton, Fla, USA, 1991.
  182. N. Shangguan, S. Katukojvala, R. Greenberg, and L. J. Williams, “The reaction of thio acids with azides: a new mechanism and new synthetic applications,” Journal of the American Chemical Society, vol. 125, no. 26, pp. 7754–7755, 2003. View at Publisher · View at Google Scholar · View at PubMed
  183. S. Drotleff, Polymers and protein-conjugates for tissue engineering, Ph.D. thesis, University of Regensburg, Regensburg, Germany, 2006.
  184. T. Hermanson, Bioconjugate Techniques, Academic Press, San Diego, Calif, USA, 1996.
  185. Y. W. Tong and M. S. Shoichet, “Peptide surface modification of poly(tetrafluoroethylene-co-hexafluoropropylene) enhances its interaction with central nervous system neurons,” Journal of Biomedical Materials Research, vol. 42, no. 1, pp. 85–95, 1998.
  186. S. P. Massia and J. A. Hubbell, “Human endothelial cell interactions with surface-coupled adhesion peptides on a nonadhesive glass substrate and two polymeric biomaterials,” Journal of Biomedical Materials Research, vol. 25, no. 2, pp. 223–242, 1991.
  187. S. P. Massia and J. A. Hubbell, “Covalently attached GRGD on polymer surfaces promotes biospecific adhesion of mammalian cells,” Annals of the New York Academy of Sciences, vol. 589, pp. 261–270, 1990. View at Publisher · View at Google Scholar
  188. K. Nilsson and K. Mosbach, “Immobilization of enzymes and affinity ligands to various hydroxyl group carrying supports using highly reactive sulfonyl chlorides,” Biochemical and Biophysical Research Communications, vol. 102, no. 1, pp. 449–457, 1981.
  189. K. Nilsson and K. Mosbach, “Tresyl chloride-activated supports for enzyme immobilization,” Methods in Enzymology, vol. 135, pp. 65–78, 1987.
  190. K. Nilsson and K. Mosbach, “Immobilization of ligands with organic sulfonyl chlorides,” Methods in Enzymology, vol. 104, pp. 56–69, 1984.
  191. R. Benters, C. M. Niemeyer, and D. Whorle, “Dendrimer-activated solid supports for nucleic acid and protein microarrays,” ChemBioChem, vol. 2, no. 9, pp. 686–694, 2001.
  192. M. Yang, R. Y. C. Kong, N. Kazmi, and A. K. C. Leung, “Covalent immobilization of oligonucleotides on modified glass/silicon surfaces for solid-phase DNA hybridization and amplification,” Chemistry Letters, no. 3, pp. 257–258, 1998.
  193. S. P. Pack, N. K. Kamisetty, M. Nonogawa, et al., “Direct immobilization of DNA oligomers onto the amine-functionalized glass surface for DNA microarray fabrication through the activation-free reaction of oxanine,” Nucleic Acids Research, vol. 35, no. 17, 2007. View at Publisher · View at Google Scholar · View at PubMed
  194. A. Dekker, et al., “Improved adhesion and proliferation of human endothelial cells on PE pre-coated with monoclonal antibodies directed against cell membrane antigens and extracellular matrix proteins,” in Adhesion and Proliferation of Human Endothelial Cells on Polymeric Surfaces, Optimization Studies, pp. 61–85, 1990.
  195. R. K. O'Reilly, M. J. Joralemon, C. J. Hawker, and K. L. Wooley, “Facile syntheses of surface-functionalized micelles and shell cross-linked nanoparticles,” Journal of Polymer Science A, vol. 44, no. 17, pp. 5203–5217, 2006. View at Publisher · View at Google Scholar
  196. K. K. Perkin, J. L. Turner, K. L. Wooley, and S. Mann, “Fabrication of hybrid nanocapsules by calcium phosphate mineralization of shell cross-linked polymer micelles and nanocages,” Nano Letters, vol. 5, no. 7, pp. 1457–1461, 2005. View at Publisher · View at Google Scholar
  197. M. L. Becker, L. A. O. Bailey, and K. L. Wooley, “Peptide-derivatized shell-cross-linked nanoparticles. 2. Biocompatibility evaluation,” Bioconjugate Chemistry, vol. 15, no. 4, pp. 710–717, 2004. View at Publisher · View at Google Scholar · View at PubMed
  198. M. L. Becker, J. Liu, and K. L. Wooley, “Peptide-polymer bioconjugates: hybrid block copolymers generated via living radical polymerizations from resin-supported peptides,” Chemical Communications, vol. 9, no. 2, pp. 180–181, 2003. View at Publisher · View at Google Scholar
  199. J. Riesle, A. P. Hollander, R. Langer, L. E. Freed, and G. Vunjak-Novakovic, “Collagen in tissue-engineered cartilage: types, structure, and crosslinks,” Journal of Cellular Biochemistry, vol. 71, no. 3, pp. 313–327, 1998. View at Publisher · View at Google Scholar
  200. J. Yang, Y. Wan, J. Yang, J. Bei, and S. Wang, “Plasma-treated, collagen-anchored polylactone: its cell affinity evaluation under shear or shear-free conditions,” Journal of Biomedical Materials Research A, vol. 67, no. 4, pp. 1139–1147, 2003.
  201. Z. Ma, C. Gao, Y. Gong, and J. Shen, “Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor,” Biomaterials, vol. 26, no. 11, pp. 1253–1259, 2005. View at Publisher · View at Google Scholar · View at PubMed
  202. S. Ber, G. Torun Köse, and V. Hasirci, “Bone tissue engineering on patterned collagen films: an in vitro study,” Biomaterials, vol. 26, no. 14, pp. 1977–1986, 2005. View at Publisher · View at Google Scholar · View at PubMed
  203. D. L. Wilson, R. Martin, S. Hong, M. Cronin-Golomb, C. A. Mirkin, and D. L. Kaplan, “Surface organization and nanopatterning of collagen by dip-pen nanolithography,” Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 24, pp. 13660–13664, 2001. View at Publisher · View at Google Scholar · View at PubMed
  204. I. Keranov, T. Vladkova, M. Minchev, A. Kostadinova, and G. Altankov, “Preparation, characterization, and cellular interactions of collagen-immobilized PDMS surfaces,” Journal of Applied Polymer Science, vol. 110, no. 1, pp. 321–330, 2008. View at Publisher · View at Google Scholar
  205. X. Liu, Y. Won, and P. X. Ma, “Surface modification of interconnected porous scaffolds,” Journal of Biomedical Materials Research A, vol. 74, no. 1, pp. 84–91, 2005. View at Publisher · View at Google Scholar · View at PubMed
  206. T. Serizawa and M. Akashi, “A novel approach for fabricating ultrathin polymer films by the repetition of the adsorption/drying processes,” Journal of Polymer Science A, vol. 37, no. 13, pp. 1903–1906, 1999.
  207. Z. Ma, C. Gao, Y. Gong, and J. Shen, “Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor,” Biomaterials, vol. 26, no. 11, pp. 1253–1259, 2005. View at Publisher · View at Google Scholar · View at PubMed
  208. M.-C. Chen, H.-F. Liang, Y.-L. Chiu, Y. Chang, H.-J. Wei, and H.-W. Sung, “A novel drug-eluting stent spray-coated with multi-layers of collagen and sirolimus,” Journal of Controlled Release, vol. 108, no. 1, pp. 178–189, 2005. View at Publisher · View at Google Scholar · View at PubMed
  209. X. Wang, H. J. Kim, P. Xu, A. Matsumoto, and D. L. Kaplan, “Biomaterial coatings by stepwise deposition of silk fibroin,” Langmuir, vol. 21, no. 24, pp. 11335–11341, 2005. View at Publisher · View at Google Scholar · View at PubMed
  210. K. Cai, K. Yao, S. Lin, et al., “Poly(D,L-lactic acid) surfaces modified by silk fibroin: effects on the culture of osteoblast in vitro,” Biomaterials, vol. 23, no. 4, pp. 1153–1160, 2002. View at Publisher · View at Google Scholar
  211. A. Chiarini, P. Petrini, S. Bozzini, I. Dal Pra, and U. Armato, “Silk fibroin/poly(carbonate)-urethane as a substrate for cell growth: in vitro interactions with human cells,” Biomaterials, vol. 24, no. 5, pp. 789–799, 2003.
  212. I. Dal Pra, P. Petrini, A. Charini, S. Bozzini, S. Farè, and U. Armato, “Silk fibroin-coated three-dimensional polyurethane scaffolds for tissue engineering: interactions with normal human fibroblasts,” Tissue Engineering, vol. 9, no. 6, pp. 1113–1121, 2003.
  213. P. Petrini, C. Parolari, and M. C. Tanzi, “Silk fibroin-polyurethane scaffolds for tissue engineering,” Journal of Materials Science, vol. 12, no. 10–12, pp. 849–853, 2001. View at Publisher · View at Google Scholar
  214. G. H. Altman, R. L. Horan, H. H. Lu, et al., “Silk matrix for tissue engineered anterior cruciate ligaments,” Biomaterials, vol. 23, no. 20, pp. 4131–4141, 2002. View at Publisher · View at Google Scholar
  215. G. H. Altman, H. H. Lu, R. L. Horan, et al., “Advanced bioreactor with controlled application of multi-dimensional strain for tissue engineering,” Journal of Biomechanical Engineering, vol. 124, no. 6, pp. 742–749, 2002. View at Publisher · View at Google Scholar
  216. J. S. Mao, H. F. Liu, Y. J. Yin, and K. D. Yao, “The properties of chitosan-gelatin membranes and scaffolds modified with hyaluronic acid by different methods,” Biomaterials, vol. 24, no. 9, pp. 1621–1629, 2003. View at Publisher · View at Google Scholar
  217. H. S. Yoo, E. A. Lee, J. J. Yoon, and T. G. Park, “Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering,” Biomaterials, vol. 26, no. 14, pp. 1925–1933, 2005. View at Publisher · View at Google Scholar · View at PubMed
  218. S. R. Hong, Y. M. Lee, and T. Akaike, “Evaluation of a galactose-carrying gelatin sponge for hepatocytes culture and transplantation,” Journal of Biomedical Materials Research A, vol. 67, no. 3, pp. 733–741, 2003.
  219. T. G. Park, “Perfusion culture of hepatocytes within galactose-derivatized biodegradable poly(lactide-co-glycolide) scaffolds prepared by gas foaming of effervescent salts,” Journal of Biomedical Materials Research, vol. 59, no. 1, pp. 127–135, 2002. View at Publisher · View at Google Scholar · View at PubMed
  220. M. P. Lutolf and J. A. Hubbell, “Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering,” Nature Biotechnology, vol. 23, no. 1, pp. 47–55, 2005. View at Publisher · View at Google Scholar · View at PubMed
  221. U. Hersel, C. Dahmen, and H. Kessler, “RGD modified polymers: biomaterials for stimulated cell adhesion and beyond,” Biomaterials, vol. 24, no. 24, pp. 4385–4415, 2003. View at Publisher · View at Google Scholar
  222. E. Ruoslahti, “RGD and other recognition sequences for integrins,” Annual Review of Cell and Developmental Biology, vol. 12, pp. 697–715, 1996. View at Publisher · View at Google Scholar · View at PubMed
  223. T. G. Kim and T. G. Park, “Biomimicking extracellular matrix: cell adhesive RGD peptide modified electrospun poly(D,L-lactic-co-glycolic acid) nanofiber mesh,” Tissue Engineering, vol. 12, no. 2, pp. 221–233, 2006. View at Publisher · View at Google Scholar · View at PubMed
  224. H. S. Mansur, Z. P. Lobato, R. L. Oréfice, W. L. Vasconcelos, C. Oliveira, and L. J. Machado, “Surface functionalization of porous glass networks: effects on bovine serum albumin and porcine insulin immobilization,” Biomacromolecules, vol. 1, no. 4, pp. 789–797, 2000.
  225. R. F. S. Lenza, J. R. Jones, W. L. Vasconcelos, and L. L. Hench, “In vitro release kinetics of proteins from bioactive foams,” Journal of Biomedical Materials Research A, vol. 67, no. 1, pp. 121–129, 2003.
  226. E. Ruoslahti, “RGD and other recognition sequences for integrins,” Annual Review of Cell and Developmental Biology, vol. 12, pp. 697–715, 1996. View at Publisher · View at Google Scholar · View at PubMed
  227. J. L. Myles, B. T. Burgess, and R. B. Dickinson, “Modification of the adhesive properties of collagen by covalent grafting with RGD peptides,” Journal of Biomaterials Science, Polymer Edition, vol. 11, no. 1, pp. 69–86, 2000.
  228. J. C. Schense, J. Bloch, P. Aebischer, and J. A. Hubbell, “Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension,” Nature Biotechnology, vol. 18, no. 4, pp. 415–419, 2000. View at Publisher · View at Google Scholar · View at PubMed
  229. S. P. Massia and J. A. Hubbell, “Human endothelial cell interactions with surface-coupled adhesion peptides on a nonadhesive glass substrate and two polymeric biomaterials,” Journal of Biomedical Materials Research, vol. 25, no. 2, pp. 223–242, 1991.
  230. M. J. Humphries, “The molecular basis and specificity of integrin-ligand interactions,” Journal of Cell Science, vol. 97, no. 4, pp. 585–592, 1990.
  231. S. P. Massia and J. A. Hubbell, Journal of Biomedical Materials Research, vol. 25, pp. 249–252, 1991.
  232. B. K. Brandley and R. L. Schnaar, “Covalent attachment of an Arg-Gly-Asp sequence peptide to derivatizable polyacrylamide surfaces: support of fibroblast adhesion and long-term growth,” Analytical Biochemistry, vol. 172, no. 1, pp. 270–278, 1988.
  233. H.-B. Lin, W. Sun, D. F. Mosher, et al., “Synthesis, surface, and cell-adhesion properties of polyurethanes containing covalently grafted RGD-peptides,” Journal of Biomedical Materials Research, vol. 28, no. 3, pp. 329–342, 1994.
  234. H.-B. Lin, Z. C. Zhao, C. Garcia-Echeverria, D. H. Rich, and S. L. Cooper, “Synthesis of a novel polyurethane co-polymer containing covalently attached RGD peptide,” Journal of Biomaterials Science, Polymer Edition, vol. 3, no. 3, pp. 217–227, 1992.
  235. P. D. Drumheller, D. L. Elbert, and J. A. Hubbell, “Multifunctional poly(ethylene glycol) semi-interpenetrating polymer networks as highly selective adhesive substrates for bioadhesive peptide grafting,” Biotechnology and Bioengineering, vol. 43, no. 8, pp. 772–780, 1994.
  236. H.-B. Lin, W. Sun, D. F. Mosher, et al., “Synthesis, surface, and cell-adhesion properties of polyurethanes containing covalently grafted RGD-peptides,” Journal of Biomedical Materials Research, vol. 28, no. 3, pp. 329–342, 1994.
  237. S. P. Massia and J. A. Hubbell, Journal of Biomedical Materials Research, vol. 25, pp. 273–282, 1991.
  238. P. D. Drumheller, D. L. Elbert, and J. A. Hubbell, “Multifunctional poly(ethylene glycol) semi-interpenetrating polymer networks as highly selective adhesive substrates for bioadhesive peptide grafting,” Biotechnology and Bioengineering, vol. 43, no. 8, pp. 772–780, 1994.
  239. J. Chen, G. H. Altman, V. Karageorgiou, et al., “Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers,” Journal of Biomedical Materials Research A, vol. 67, no. 2, pp. 559–570, 2003.
  240. Y.-S. Lin, S.-S. Wang, T.-W. Chung, et al., “Growth of endothelial cells on different concentrations of Gly-Arg-Gly-Asp photochemically grafted in polyethylene glycol modified polyurethane,” Artificial Organs, vol. 25, no. 8, pp. 617–621, 2001. View at Publisher · View at Google Scholar
  241. L. De Bartolo, S. Morelli, A. Piscioneri, et al., “Novel membranes and surface modification able to activate specific cellular responses,” Biomolecular Engineering, vol. 24, no. 1, pp. 23–26, 2007. View at Publisher · View at Google Scholar · View at PubMed
  242. S. S. Lateef, S. Boateng, T. J. Hartman, C. A. Crot, B. Russell, and L. Hanley, “GRGDSP peptide-bound silicone membranes withstand mechanical flexing in vitro and display enhanced fibroblast adhesion,” Biomaterials, vol. 23, no. 15, pp. 3159–3168, 2002. View at Publisher · View at Google Scholar
  243. Y. Hu, S. R. Winn, I. Krajbich, and J. O. Hollinger, “Porous polymer scaffolds surface-modified with arginine-glycine-aspartic acid enhance bone cell attachment and differentiation in vitro,” Journal of Biomedical Materials Research A, vol. 64, no. 3, pp. 583–590, 2003.
  244. M.-H. Ho, L.-T. Hou, C.-Y. Tu, et al., “Promotion of cell affinity of porous PLLA scaffolds by immobilization of RGD peptides via plasma treatment,” Macromolecular Bioscience, vol. 6, no. 1, pp. 90–98, 2006. View at Publisher · View at Google Scholar · View at PubMed
  245. D. S. W. Benoit and K. S. Anseth, “The effect on osteoblast function of colocalized RGD and PHSRN epitopes on PEG surfaces,” Biomaterials, vol. 26, no. 25, pp. 5209–5220, 2005. View at Publisher · View at Google Scholar · View at PubMed
  246. D. L. Elbert and J. A. Hubbell, “Surface treatments of polymers for biocompatibility,” Annual Review of Materials Science, vol. 26, no. 1, pp. 365–394, 1996.
  247. J. P. Tam, Q. Yu, and Z. Miao, “Orthogonal ligation strategies for peptide and protein,” Biopolymers, vol. 51, no. 5, pp. 311–332, 1999. View at Publisher · View at Google Scholar
  248. J. A. Camarero, Y. Kwon, and M. A. Coleman, “Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for “protein chip” fabrication,” Journal of the American Chemical Society, vol. 126, no. 45, pp. 14730–14731, 2004. View at Publisher · View at Google Scholar · View at PubMed
  249. G. Thumshirn, U. Hersel, S. L. Goodman, and H. Kessler, “Multimeric cyclic RGD peptides as potential tools for tumor targeting: solid-phase peptide synthesis and chemoselective oxime ligation,” Chemistry, vol. 9, no. 12, pp. 2717–2725, 2003. View at Publisher · View at Google Scholar · View at PubMed
  250. L. Scheibler, P. Dumy, M. Boncheva, et al., “Functional molecular thin films: topological templates for the chemoselective ligation of antigenic peptides to self-assembled monolayers,” Angewandte Chemie: International Edition, vol. 38, no. 5, pp. 696–699, 1999. View at Publisher · View at Google Scholar
  251. S. P. Massia and J. A. Hubbell, “Immobilized amines and basic amino acids as mimetic heparin-binding domains for cell surface proteoglycan-mediated adhesion,” Journal of Biological Chemistry, vol. 267, no. 14, pp. 10133–10141, 1992.
  252. W.-J. Li, R. Tuli, X. Huang, P. Laquerriere, and R. S. Tuan, “Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold,” Biomaterials, vol. 26, no. 25, pp. 5158–5166, 2005. View at Publisher · View at Google Scholar · View at PubMed
  253. W.-J. Li, C. T. Laurencin, E. J. Caterson, R. S. Tuan, and F. K. Ko, “Electrospun nanofibrous structure: a novel scaffold for tissue engineering,” Journal of Biomedical Materials Research, vol. 60, no. 4, pp. 613–621, 2002. View at Publisher · View at Google Scholar · View at PubMed
  254. A. Mata, C. Boehm, A. J. Fleischman, G. Muschler, and S. Roy, “Analysis of connective tissue progenitor cell behavior on polydimethylsiloxane smooth and channel micro-textures,” Biomedical Microdevices, vol. 4, no. 4, pp. 267–275, 2002. View at Publisher · View at Google Scholar
  255. G. Assero, C. Satriano, G. Lupo, C. D. Anfuso, G. Marletta, and M. Alberghina, “Pericyte adhesion and growth onto polyhydroxymethylsiloxane surfaces nanostructured by plasma treatment and ion irradiation,” Microvascular Research, vol. 68, no. 3, pp. 209–220, 2004. View at Publisher · View at Google Scholar · View at PubMed
  256. R. Raman, G. Venkataraman, S. Ernst, V. Sasisekharan, and R. Sasisekharan, “Structural specificity of heparin binding in the fibroblast growth factor family of proteins,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 5, pp. 2357–2362, 2003. View at Publisher · View at Google Scholar · View at PubMed
  257. M. J. B. Wissink, R. Beernink, A. A. Poot, et al., “Improved endothelialization of vascular grafts by local release of growth factor from heparinized collagen matrices,” Journal of Controlled Release, vol. 64, no. 1–3, pp. 103–114, 2000. View at Publisher · View at Google Scholar
  258. J. S. Pieper, T. Hafmans, P. B. Van Wachem, et al., “Loading of collagen-heparan sulfate matrices with bFGF promotes angiogenesis and tissue generation in rats,” Journal of Biomedical Materials Research, vol. 62, no. 2, pp. 185–194, 2002. View at Publisher · View at Google Scholar · View at PubMed
  259. S. E. Sakiyama-Elbert and J. A. Hubbell, “Development of fibrin derivatives for controlled release of heparin-binding growth factors,” Journal of Controlled Release, vol. 65, no. 3, pp. 389–402, 2000. View at Publisher · View at Google Scholar
  260. J. Li, J. Pan, L. Zhang, X. Guo, and Y. Yu, “Culture of primary rat hepatocytes within porous chitosan scaffolds,” Journal of Biomedical Materials Research A, vol. 67, no. 3, pp. 938–943, 2003.
  261. A. T. Gutsche, H. Lo, J. Zurlo, J. Yager, and K. W. Leong, “Engineering of a sugar-derivatized porous network for hepatocyte culture,” Biomaterials, vol. 17, no. 3, pp. 387–393, 1996. View at Publisher · View at Google Scholar
  262. J. J. Yoon, H. J. Chung, H. J. Lee, and T. G. Park, “Heparin-immobilized biodegradable scaffolds for local and sustained release of angiogenic growth factor,” Journal of Biomedical Materials Research A, vol. 79, no. 4, pp. 934–942, 2006. View at Publisher · View at Google Scholar · View at PubMed
  263. H. J. Chung, H. K. Kim, J. J. Yoon, and T. G. Park, “Heparin immobilized porous PLGA microspheres for angiogenic growth factor delivery,” Pharmaceutical Research, vol. 23, no. 8, pp. 1835–1841, 2006. View at Publisher · View at Google Scholar · View at PubMed
  264. D. D. Hile, M. L. Amirpour, A. Akgerman, and M. V. Pishko, “Active growth factor delivery from poly(D,L-lactide-co-glycolide) foams prepared in supercritical CO2,” Journal of Controlled Release, vol. 66, no. 2-3, pp. 177–185, 2000. View at Publisher · View at Google Scholar
  265. H. Lee, R. A. Cusick, F. Browne, et al., “Local delivery of basic fibroblast growth factor increases both angiogenesis and engraftment of hepatocytes in tissue-engineered polymer devices,” Transplantation, vol. 73, no. 10, pp. 1589–1593, 2002.
  266. R. Padera, G. Venkataraman, D. Berry, R. Godavarti, and R. Sasisekharan, “FGF-2/fibroblast growth factor receptor/heparin-like glycosaminoglycan interactions: a compensation model for FGF-2 signaling,” FASEB Journal, vol. 13, no. 13, pp. 1677–1687, 1999.
  267. L. D. Thompson, M. Pantoliano, and B. Springer, “Energetic characterization of the basic fibroblast growth factor-heparin interaction: identification of the heparin binding domain,” Biochemistry, vol. 33, no. 13, pp. 3831–3840, 1994.
  268. R. Raman, G. Venkataraman, S. Ernst, V. Sasisekharan, and R. Sasisekharan, “Structural specificity of heparin binding in the fibroblast growth factor family of proteins,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 5, pp. 2357–2362, 2003. View at Publisher · View at Google Scholar · View at PubMed
  269. J. J. Yoon, H. J. Chung, H. J. Lee, and T. G. Park, “Heparin-immobilized biodegradable scaffolds for local and sustained release of angiogenic growth factor,” Journal of Biomedical Materials Research A, vol. 79, no. 4, pp. 934–942, 2006. View at Publisher · View at Google Scholar · View at PubMed
  270. H. K. Kim, H. J. Chung, and T. G. Park, “Biodegradable polymeric microspheres with “open/closed” pores for sustained release of human growth hormone,” Journal of Controlled Release, vol. 112, no. 2, pp. 167–174, 2006. View at Publisher · View at Google Scholar · View at PubMed
  271. H. Lee, H. J. Chung, and T. G. Park, “Perspectives on: local and sustained delivery of angiogenic growth factors,” Journal of Bioactive and Compatible Polymers, vol. 22, no. 1, pp. 89–114, 2007. View at Publisher · View at Google Scholar
  272. C. P. Vepari and D. L. Kaplan, “Covalently immobilized enzyme gradients within three-dimensional porous scaffolds,” Biotechnology and Bioengineering, vol. 93, no. 6, pp. 1130–1137, 2006. View at Publisher · View at Google Scholar · View at PubMed
  273. M. Wang, “Surface modification of biomaterials and tissue engineering scaffolds for enhanced osteoconductivity,” in Proceedings of the 3rd Kuala Lumpur International Conference on Biomedical Engineering, vol. 15, pp. 22–27, Kuala Lumpur, Malaysia, December 2006.
  274. Z. Hu, Y. Chen, C. Wang, Y. Zheng, and Y. Li, “Polymer gels with engineered environmentally responsive surface patterns,” Nature, vol. 393, no. 6681, pp. 149–152, 1998. View at Publisher · View at Google Scholar
  275. M. Ebara, J. M. Hoffman, A. S. Hoffman, and P. S. Stayton, “Switchable surface traps for injectable bead-based chromatography in PDMS microfluidic channels,” Lab on a Chip, vol. 6, no. 7, pp. 843–848, 2006. View at Publisher · View at Google Scholar · View at PubMed
  276. F. Stoica, C. Alexander, N. Tirelli, A. F. Miller, and A. Saiani, “Selective synthesis of double temperature-sensitive polymer-peptide conjugates,” Chemical Communications, no. 37, pp. 4433–4435, 2008. View at Publisher · View at Google Scholar · View at PubMed