Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model

Table 2

Media and incubation conditions used for enumeration of representative microbes in the simulated human colon.

Microbial group	Medium	Incubation conditions and time	Colonies formed
Total aerobes	Brain heart infusion agar	Aerobic, 37^∘C, 24 hours	White
Total anaerobes	Brain heart infusion agar	Anaerobic, 37^∘C, 72 hours	White
Escherichia coli	Mc Conkey agar	Aerobic, 43^∘C, 24 hours	Red-purple
Staphylococcus sp.	Mannitol Salt agar	Aerobic, 37^∘C, 48 hours	White with yellow/purple zone
Lactobacillus sp.	Rogosa agar	Anaerobic, 37^∘C, 72 hours	White