Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model
Table 3
Effects of microcapsules on fecal marker microbes in the simulated transverse colon.
Microbes
Incubation time (h)
Log CFU/mL medium
GCAC
AC
Controlc
Total aerobes
0
8.41
8.41
8.41
6
8.37
8.32
8.36
12
8.36
8.37
8.39
24
8.22
8.17
8.04
Total anaerobes
0
8.44
8.44
8.44
6
8.37
8.33
8.41
12
8.37
8.43
8.40
24
8.41
8.30
8.03
Escherichia coli
0
8.31
8.31
8.31
6
8.18
7.97
8.18
12
8.20
8.02
8.41
24
8.10
8.03
8.68
Staphylococcus sp.
0
6.96
6.96
6.96
6
6.52
6.60
6.81
12
6.61
6.72
6.77
24
6.57
6.62
6.54
Lactobacillus sp.
0
5.48
5.48
5.48
6
5.45
5.52
5.51
12
5.35
5.40
5.32
24
5.50
5.46
5.37
n = 3, standard deviation 0.20. Colonic suspension in the presence of microcapsules. Colonic suspension in the absence of microcapsules.