Research Article

Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model

Table 3

Effects of microcapsules on fecal marker microbes in the simulated transverse colon.

MicrobesIncubation time (h)Log CFU/mL medium
GCAC AC Controlc

Total aerobes08.418.418.41
68.378.328.36
128.368.378.39
248.228.178.04

Total anaerobes08.448.448.44
68.378.338.41
128.378.438.40
248.418.308.03

Escherichia coli08.318.318.31
68.187.978.18
128.208.028.41
248.108.038.68

Staphylococcus sp.06.966.966.96
66.526.606.81
126.616.726.77
246.576.626.54

Lactobacillus sp.05.485.485.48
65.455.525.51
125.355.405.32
245.505.465.37

n = 3, standard deviation 0.20.
Colonic suspension in the presence of microcapsules.
Colonic suspension in the absence of microcapsules.