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International Journal of Polymer Science
Volume 2012 (2012), Article ID 174942, 25 pages
doi:10.1155/2012/174942
Scaffolds for Growth Factor Delivery as Applied to Bone Tissue Engineering
1Biomaterials and Tissue Morphology Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, Australia
2Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, Australia
3Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, Australia
Received 21 April 2012; Revised 3 September 2012; Accepted 25 September 2012
Academic Editor: Christopher Batich
Copyright © 2012 Keith A. Blackwood et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Linked References
- R. Burge, B. Dawson-Hughes, D. H. Solomon, J. B. Wong, A. King, and A. Tosteson, “Incidence and economic burden of osteoporosis-related fractures in the United States, 2005–2025,” Journal of Bone and Mineral Research, vol. 22, no. 3, pp. 465–475, 2007. View at Publisher · View at Google Scholar · View at Scopus
- K. M. Sanders, G. C. Nicholson, A. M. Ugoni, J. A. Pasco, E. Seeman, and M. A. Kotowicz, “Health burden of hip and other fractures in Australia beyond 2000. Projections based on the Geelong Osteoporosis Study,” Medical Journal of Australia, vol. 170, no. 10, pp. 467–470, 1999. View at Scopus
- N. Ferrara, H. P. Gerber, and J. LeCouter, “The biology of VEGF and its receptors,” Nature Medicine, vol. 9, no. 6, pp. 669–676, 2003. View at Publisher · View at Google Scholar · View at Scopus
- T. Katagiri, A. Yamaguchi, M. Komaki et al., “Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage,” The Journal of Cell Biology, vol. 127, no. 6, pp. 1755–1766, 1994. View at Publisher · View at Google Scholar · View at Scopus
- M. Inada, T. Katagiri, S. Akiyama et al., “Bone morphogenetic protein-12 and -13 inhibit terminal differentiation of myoblasts, but do not induce their differentiation into osteoblasts,” Biochemical and Biophysical Research Communications, vol. 222, no. 2, pp. 317–322, 1996. View at Publisher · View at Google Scholar · View at Scopus
- C. A. Kirker-Head, “Potential applications and delivery strategies for bone morphogenetic proteins,” Advanced Drug Delivery Reviews, vol. 43, no. 1, pp. 65–92, 2000. View at Publisher · View at Google Scholar · View at Scopus
- M. M. L. Deckers, R. L. van Bezooijen, G. van der Horst et al., “Bone morphogenetic proteins stimulate angiogenesis through osteoblast-derived vascular endothelial growth factor A,” Endocrinology, vol. 143, no. 4, pp. 1545–1553, 2002. View at Publisher · View at Google Scholar · View at Scopus
- J. Fiedler, G. Röderer, K. P. Günther, and R. E. Brenner, “BMP-2, BMP-4, and PDGF-bb stimulate chemotactic migration of primary human mesenchymal progenitor cells,” Journal of Cellular Biochemistry, vol. 87, no. 3, pp. 305–312, 2002. View at Publisher · View at Google Scholar · View at Scopus
- W. Friess, H. Uludag, S. Foskett, R. Biron, and C. Sargeant, “Characterization of absorbable collagen sponges as rhBMP-2 carriers,” International Journal of Pharmaceutics, vol. 187, no. 1, pp. 91–99, 1999. View at Publisher · View at Google Scholar · View at Scopus
- J. O. Hollinger, J. M. Schmitt, D. C. Buck et al., “Recombinant human bone morphogenetic protein-2 and collagen for bone regeneration,” Journal of Biomedical Materials Research, vol. 43, no. 4, pp. 356–364, 1998.
- E. J. Carragee, E. L. Hurwitz, and B. K. Weiner, “A critical review of recombinant human bone morphogenetic protein-2 trials in spinal surgery: emerging safety concerns and lessons learned,” Spine Journal, vol. 11, no. 6, pp. 471–491, 2011. View at Publisher · View at Google Scholar · View at Scopus
- R. Vasita and D. S. Katti, “Growth factor-delivery systems for tissue engineering: a materials perspective,” Expert Review of Medical Devices, vol. 3, no. 1, pp. 29–47, 2006. View at Publisher · View at Google Scholar · View at Scopus
- R. R. Chen and D. J. Mooney, “Polymeric growth factor delivery strategies for tissue engineering,” Pharmaceutical Research, vol. 20, no. 8, pp. 1103–1112, 2003. View at Publisher · View at Google Scholar · View at Scopus
- J. R. Porter, T. T. Ruckh, and K. C. Popat, “Bone tissue engineering: a review in bone biomimetics and drug delivery strategies,” Biotechnology Progress, vol. 25, no. 6, pp. 1539–1560, 2009. View at Publisher · View at Google Scholar · View at Scopus
- M. Noda, Cellular and Molecular Biology of Bone, Academic Press, New York, NY, USA, 1993.
- J. C. Reichert, S. Saifzadeh, M. E. Wullschleger et al., “The challenge of establishing preclinical models for segmental bone defect research,” Biomaterials, vol. 30, no. 12, pp. 2149–2163, 2009. View at Publisher · View at Google Scholar · View at Scopus
- C. Szpalski, J. Barr, M. Wetterau, P. B. Saadeh, and S. M. Warren, “Cranial bone defects: current and future strategies,” Neurosurgical Focus, vol. 29, no. 6, p. E8, 2010. View at Scopus
- A. R. Vaccaro, K. Singh, R. Haid et al., “The use of bioabsorbable implants in the spine,” Spine Journal, vol. 3, no. 3, pp. 227–237, 2003. View at Publisher · View at Google Scholar · View at Scopus
- P. N. Soucacos, E. O. Johnson, and G. Babis, “An update on recent advances in bone regeneration,” Injury, vol. 39, supplement 2, pp. S1–S4, 2008. View at Publisher · View at Google Scholar · View at Scopus
- E. M. Younger and M. W. Chapman, “Morbidity at bone graft donor sites,” Journal of orthopaedic trauma, vol. 3, no. 3, pp. 192–195, 1989. View at Scopus
- R. Dimitriou, G. I. Mataliotakis, A. G. Angoules, N. K. Kanakaris, and P. V. Giannoudis, “Complications following autologous bone graft harvesting from the iliac crest and using the RIA: a systematic review,” Injury, vol. 42, supplement 2, pp. S3–S15, 2011. View at Publisher · View at Google Scholar · View at Scopus
- B. A. Rogers, A. Sternheim, D. Backstein, O. Safir, and A. E. Gross, “Proximal femoral allograft for major segmental femoral bone loss: a systematic literature review,” Advances in Orthopedics, vol. 2011, Article ID 257572, 7 pages, 2011.
- M. A. Woodruff and D. W. Hutmacher, “The return of a forgotten polymer—polycaprolactone in the 21st century,” Progress in Polymer Science, vol. 35, no. 10, pp. 1217–1256, 2010. View at Publisher · View at Google Scholar · View at Scopus
- M. Geiger, R. H. Li, and W. Friess, “Collagen sponges for bone regeneration with rhBMP-2,” Advanced Drug Delivery Reviews, vol. 55, no. 12, pp. 1613–1629, 2003. View at Publisher · View at Google Scholar · View at Scopus
- Y. M. Kolambkar, K. M. Dupont, J. D. Boerckel et al., “An alginate-based hybrid system for growth factor delivery in the functional repair of large bone defects,” Biomaterials, vol. 32, no. 1, pp. 65–74, 2011. View at Publisher · View at Google Scholar · View at Scopus
- Y. Gu, L. Chen, H. L. Yang, Z. P. Luo, and T. S. Tang, “Evaluation of an injectable silk fibroin enhanced calcium phosphate cement loaded with human recombinant bone morphogenetic protein-2 in ovine lumbar interbody fusion,” Journal of Biomedical Materials Research A, vol. 97, no. 2, pp. 177–185, 2011. View at Publisher · View at Google Scholar · View at Scopus
- E. S. Place, N. D. Evans, and M. M. Stevens, “Complexity in biomaterials for tissue engineering,” Nature Materials, vol. 8, no. 6, pp. 457–470, 2009. View at Publisher · View at Google Scholar · View at Scopus
- M. Vert, S. M. Li, G. Spenlehauer, and P. Guerin, “Bioresorbability and biocompatibility of aliphatic polyesters,” Journal of Materials Science, vol. 3, no. 6, pp. 432–446, 1992. View at Publisher · View at Google Scholar · View at Scopus
- A. U. Daniels, M. K. Chang, and K. P. Andriano, “Mechanical properties of biodegradable polymers and composites proposed for internal fixation of bone,” Journal of Applied Biomaterials, vol. 1, no. 1, pp. 57–78, 1990. View at Scopus
- R. K. Kulkarni, K. C. Pani, C. Neuman, and F. Leonard, “Polylactic acid for surgical implants,” Archives of Surgery, vol. 93, no. 5, pp. 839–843, 1966. View at Scopus
- J. Y. Paeng, J. Hong, C. S. Kim, and M. J. Kim, “Comparative study of skeletal stability between bicortical resorbable and titanium screw fixation after sagittal split ramus osteotomy for mandibular prognathism,” Journal of Cranio-Maxillofacial Surgery. In press. View at Publisher · View at Google Scholar
- R. E. Holmes, S. R. Cohen, G. B. Cornwall, K. A. Thomas, K. K. Kleinhenz, and M. Z. Beckett, “MacroPore resorbable devices in craniofacial surgery,” Clinics in Plastic Surgery, vol. 31, no. 3, pp. 393–406, 2004. View at Publisher · View at Google Scholar · View at Scopus
- N. Ashammakhi and P. Rokkanen, “Absorbable polyglycolide devices in trauma and bone surgery,” Biomaterials, vol. 18, no. 1, pp. 3–9, 1997. View at Publisher · View at Google Scholar · View at Scopus
- B. C. Kim, B. L. Padwa, H. S. Park, and Y. S. Jung, “Stability of maxillary position after le fort i osteotomy using self-reinforced biodegradable poly-70L/30DL-lactide miniplates and screws,” Journal of Oral and Maxillofacial Surgery, vol. 69, no. 5, pp. 1442–1446, 2011. View at Publisher · View at Google Scholar · View at Scopus
- M. Kamitakahara, C. Ohtsuki, and T. Miyazaki, “Behavior of ceramic biomaterials derived from tricalcium phosphate in physiological condition,” Journal of Biomaterials Applications, vol. 23, no. 3, pp. 197–212, 2008. View at Publisher · View at Google Scholar · View at Scopus
- E. S. Place, L. Rojo, E. Gentleman, J. P. Sardinha, and M. M. Stevens, “Strontium-and zinc-alginate hydrogels for bone tissue engineering,” Tissue Engineering A, vol. 17, no. 21-22, pp. 2713–2722, 2011. View at Publisher · View at Google Scholar · View at Scopus
- Z. S. Haidar, R. C. Hamdy, and M. Tabrizian, “Delivery of recombinant bone morphogenetic proteins for bone regeneration and repair. Part B: delivery systems for BMPs in orthopaedic and craniofacial tissue engineering,” Biotechnology Letters, vol. 31, no. 12, pp. 1825–1835, 2009. View at Publisher · View at Google Scholar · View at Scopus
- D. Williams, “Revisiting the definition of biocompatibility,” Medical Device Technology, vol. 14, no. 8, pp. 10–13, 2003. View at Scopus
- D. Chen, M. Zhao, and G. R. Mundy, “Bone morphogenetic proteins,” Growth Factors, vol. 22, no. 4, pp. 233–241, 2004. View at Publisher · View at Google Scholar · View at Scopus
- A. H. Reddi, “Bone morphogenetic proteins: from basic science to clinical applications,” Journal of Bone and Joint Surgery A, vol. 83, supplement 1, part 1, pp. S1–S6, 2001. View at Scopus
- M. I. Menendez, D. J. Clark, M. Carlton et al., “Direct delayed human adenoviral BMP-2 or BMP-6 gene therapy for bone and cartilage regeneration in a pony osteochondral model,” Osteoarthritis and Cartilage, vol. 19, no. 8, pp. 1066–1075, 2011. View at Publisher · View at Google Scholar · View at Scopus
- J. H. Christiansen, E. G. Coles, and D. G. Wilkinson, “Molecular control of neural crest formation, migration and differentiation,” Current Opinion in Cell Biology, vol. 12, no. 6, pp. 719–724, 2000. View at Publisher · View at Google Scholar · View at Scopus
- R. W. Haid Jr., C. L. Branch Jr., J. T. Alexander, and J. K. Burkus, “Posterior lumbar interbody fusion using recombinant human bone morphogenetic protein type 2 with cylindrical interbody cages,” Spine Journal, vol. 4, no. 5, pp. 527–538, 2004. View at Publisher · View at Google Scholar · View at Scopus
- Z. S. Haidar, R. C. Hamdy, and M. Tabrizian, “Delivery of recombinant bone morphogenetic proteins for bone regeneration and repair. Part A: current challenges in BMP delivery,” Biotechnology Letters, vol. 31, no. 12, pp. 1817–1824, 2009. View at Publisher · View at Google Scholar · View at Scopus
- D. H. R. Kempen, L. Lu, A. Heijink et al., “Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration,” Biomaterials, vol. 30, no. 14, pp. 2816–2825, 2009. View at Publisher · View at Google Scholar · View at Scopus
- J. D. Boerckel, Y. M. Kolambkar, K. M. Dupont et al., “Effects of protein dose and delivery system on BMP-mediated bone regeneration,” Biomaterials, vol. 32, no. 22, pp. 5241–5251, 2011. View at Publisher · View at Google Scholar · View at Scopus
- Q. Li, T. Hou, J. Zhao, and J. Xu, “Vascular endothelial growth factor release from alginate microspheres under simulated physiological compressive loading and the effect on human vascular endothelial cells,” Tissue Engineering A, vol. 17, no. 13-14, pp. 1777–1785, 2011. View at Publisher · View at Google Scholar · View at Scopus
- A. Lochmann, H. Nitzsche, S. von Einem, E. Schwarz, and K. Mäder, “The influence of covalently linked and free polyethylene glycol on the structural and release properties of rhBMP-2 loaded microspheres,” Journal of Controlled Release, vol. 147, no. 1, pp. 92–100, 2010. View at Publisher · View at Google Scholar · View at Scopus
- J. Patterson, R. Siew, S. W. Herring, A. S. P. Lin, R. Guldberg, and P. S. Stayton, “Hyaluronic acid hydrogels with controlled degradation properties for oriented bone regeneration,” Biomaterials, vol. 31, no. 26, pp. 6772–6781, 2010. View at Publisher · View at Google Scholar · View at Scopus
- N. Bock, M. A. Woodruff, D. W. Hutmacher, and T. R. Dargaville, “Electrospraying, a reproducible method for production of polymeric microspheres for biomedical applications,” Polymers, vol. 3, no. 1, pp. 131–149, 2011. View at Publisher · View at Google Scholar · View at Scopus
- A. Sukarto and B. G. Amsden, “Low melting point amphiphilic microspheres for delivery of bone morphogenetic protein-6 and transforming growth factor-β3 in a hydrogel matrix,” Journal of Controlled Release, vol. 158, no. 1, pp. 53–62, 2012. View at Publisher · View at Google Scholar · View at Scopus
- L. Solorio, C. Zwolinski, A. W. Lund, M. J. Farrell, and J. P. Stegemann, “Gelatin microspheres crosslinked with genipin for local delivery of growth factors,” Journal of Tissue Engineering and Regenerative Medicine, vol. 4, no. 7, pp. 514–523, 2010. View at Scopus
- D. H. R. Kempen, L. Lu, T. E. Hefferan et al., “Retention of in vitro and in vivo BMP-2 bioactivities in sustained delivery vehicles for bone tissue engineering,” Biomaterials, vol. 29, no. 22, pp. 3245–3252, 2008. View at Publisher · View at Google Scholar · View at Scopus
- S. Chen, A. Osaka, T. Ikoma et al., “Fabrication, microstructure, and BMP-2 delivery of novel biodegradable and biocompatible silicate-collagen hybrid fibril sheets,” Journal of Materials Chemistry, vol. 21, no. 29, pp. 10942–10948, 2011. View at Publisher · View at Google Scholar · View at Scopus
- E. Martínez-Sanz, D. A. Ossipov, J. Hilborn, S. Larsson, K. B. Jonsson, and O. P. Varghese, “Bone reservoir: injectable hyaluronic acid hydrogel for minimal invasive bone augmentation,” Journal of Controlled Release, vol. 152, no. 2, pp. 232–240, 2011. View at Publisher · View at Google Scholar · View at Scopus
- T. Jiang, Y. Khan, L. S. Nair, W. I. Abdel-Fattah, and C. T. Laurencin, “Functionalization of chitosan/poly(lactic acid-glycolic acid) sintered microsphere scaffolds via surface heparinization for bone tissue engineering,” Journal of Biomedical Materials Research A, vol. 93, no. 3, pp. 1193–1208, 2010. View at Publisher · View at Google Scholar · View at Scopus
- G. J. Dawes, L. E. Fratila-Apachitei, B. S. Necula et al., “Effects of dexamethasone loaded plga microspheres on human fetal osteoblasts,” Journal of Biomaterials Applications, vol. 27, no. 4, pp. 477–483, 2012.
- P. C. Bessa, E. R. Balmayor, H. S. Azevedo et al., “Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release,” Journal of Tissue Engineering and Regenerative Medicine, vol. 4, no. 5, pp. 349–355, 2010. View at Publisher · View at Google Scholar · View at Scopus
- M. R. Johnson, H. J. Lee, R. V. Bellamkonda, and R. E. Guldberg, “Sustained release of BMP-2 in a lipid-based microtube vehicle,” Acta Biomaterialia, vol. 5, no. 1, pp. 23–28, 2009. View at Publisher · View at Google Scholar · View at Scopus
- T. M. Filion, A. Kutikov, and J. Song, “Chemically modified cellulose fibrous meshes for use as tissue engineering scaffolds,” Bioorganic and Medicinal Chemistry Letters, vol. 21, no. 17, pp. 5067–5070, 2011. View at Publisher · View at Google Scholar · View at Scopus
- G. T. S. Kirby, L. J. White, C. V. Rahman et al., “PLGA-based microparticles for the sustained release of BMP-2,” Polymers, vol. 3, no. 1, pp. 571–586, 2011. View at Publisher · View at Google Scholar
- J. P. Schmitz and J. O. Hollinger, “The critical size defect as an experimental model for craniomandibulofacial nonunions,” Clinical Orthopaedics and Related Research, vol. 205, no. 205, pp. 299–308, 1986. View at Scopus
- Y. Ji, G. P. Xu, Z. P. Zhang, J. J. Xia, J. L. Yan, and S. H. Pan, “BMP-2/PLGA delayed-release microspheres composite graft, selection of bone particulate diameters, and prevention of aseptic inflammation for bone tissue engineering,” Annals of Biomedical Engineering, vol. 38, no. 3, pp. 632–639, 2010. View at Publisher · View at Google Scholar · View at Scopus
- G. Hulsart-Billström, Q. Hu, K. Bergman et al., “Calcium phosphates compounds in conjunction with hydrogel as carrier for BMP-2: a study on ectopic bone formation in rats,” Acta Biomaterialia, vol. 7, no. 8, pp. 3042–3049, 2011. View at Publisher · View at Google Scholar · View at Scopus
- M. Larsen, W. F. Willems, M. Pelzer, P. F. Friedrich, M. J. Yaszemski, and A. T. Bishop, “Augmentation of surgical angiogenesis in vascularized bone allotransplants with host-derived A/V bundle implantation, fibroblast growth factor-2, and vascular endothelial growth factor administration,” Journal of Orthopaedic Research, vol. 28, no. 8, pp. 1015–1021, 2010. View at Publisher · View at Google Scholar · View at Scopus
- S. N. Rath, G. Pryymachuk, O. A. Bleiziffer et al., “Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: invitro and invivo evaluation in a PLDLLA-TCP-PCL-composite system,” Journal of Materials Science, vol. 22, no. 5, pp. 1279–1291, 2011. View at Publisher · View at Google Scholar · View at Scopus
- W. F. Willems, M. Larsen, G. Giusti, P. F. Friedrich, and A. T. Bishop, “Revascularization and bone remodeling of frozen allografts stimulated by intramedullary sustained delivery of FGF-2 and VEGF,” Journal of Orthopaedic Research, vol. 29, no. 9, pp. 1431–1436, 2011. View at Publisher · View at Google Scholar · View at Scopus
- M. D. Schofer, P. P. Roessler, J. Schaefer et al., “Electrospun plla nanofiber scaffolds and their use in combination with bmp-2 for reconstruction of bone defects,” PLoS One, vol. 6, no. 9, Article ID e25462, 2011. View at Publisher · View at Google Scholar · View at Scopus
- A. Suzuki, H. Terai, H. Toyoda et al., “A biodegradable delivery system for antibiotics and recombinant human bone morphogenetic protein-2: a potential treatment for infected bone defects,” Journal of Orthopaedic Research, vol. 24, no. 3, pp. 327–332, 2006. View at Publisher · View at Google Scholar · View at Scopus
- S. Young, Z. S. Patel, J. D. Kretlow et al., “Dose effect of dual delivery of vascular endothelial growth factor and bone morphogenetic protein-2 on bone regeneration in a rat critical-size defect model,” Tissue Engineering A, vol. 15, no. 9, pp. 2347–2362, 2009. View at Publisher · View at Google Scholar · View at Scopus
- Z. X. Wu, D. Liu, S. Y. Wan, G. Cui, Y. Zhang, and W. Lei, “Sustained-release rhBMP-2 increased bone mass and bone strength in an ovine model of postmenopausal osteoporosis,” Journal of Orthopaedic Science, vol. 16, no. 1, pp. 99–104, 2011. View at Publisher · View at Google Scholar · View at Scopus
- M. Li, X. Liu, X. Liu, and B. Ge, “Calcium phosphate cement with BMP-2-loaded gelatin microspheres enhances bone healing in osteoporosis: a pilot study,” Clinical Orthopaedics and Related Research, vol. 468, no. 7, pp. 1978–1985, 2010. View at Publisher · View at Google Scholar · View at Scopus
- Y. M. Kolambkar, J. D. Boerckel, K. M. Dupont et al., “Spatiotemporal delivery of bone morphogenetic protein enhances functional repair of segmental bone defects,” Bone, vol. 49, no. 3, pp. 485–492, 2011. View at Publisher · View at Google Scholar · View at Scopus
- C. Kirker-Head, V. Karageorgiou, S. Hofmann et al., “BMP-silk composite matrices heal critically sized femoral defects,” Bone, vol. 41, no. 2, pp. 247–255, 2007. View at Publisher · View at Google Scholar · View at Scopus
- K. V. Brown, B. Li, T. Guda, D. S. Perrien, S. A. Guelcher, and J. C. Wenke, “Improving bone formation in a rat femur segmental defect by controlling bone morphogenetic protein-2 release,” Tissue Engineering A, vol. 17, no. 13-14, pp. 1735–1746, 2011. View at Publisher · View at Google Scholar · View at Scopus
- O. Jeon, S. J. Song, S. W. Kang, A. J. Putnam, and B. S. Kim, “Enhancement of ectopic bone formation by bone morphogenetic protein-2 released from a heparin-conjugated poly(l-lactic-co-glycolic acid) scaffold,” Biomaterials, vol. 28, no. 17, pp. 2763–2771, 2007. View at Publisher · View at Google Scholar · View at Scopus
- O. Jeon, J. W. Rhie, I. K. Kwon, J. H. Kim, B. S. Kim, and S. H. Lee, “In vivo bone formation following transplantation of human adipose-derived stromal cells that are not differentiated osteogenically,” Tissue Engineering A, vol. 14, no. 8, pp. 1285–1294, 2008. View at Publisher · View at Google Scholar · View at Scopus
- A. M. Henslee, P. P. Spicer, D. M. Yoon et al., “Biodegradable composite scaffolds incorporating an intramedullary rod and delivering bone morphogenetic protein-2 for stabilization and bone regeneration in segmental long bone defects,” Acta Biomaterialia, vol. 7, no. 10, pp. 3627–3637, 2011. View at Publisher · View at Google Scholar · View at Scopus
- L. Luca, A. L. Rougemont, B. H. Walpoth et al., “Injectable rhBMP-2-loaded chitosan hydrogel composite: Osteoinduction at ectopic site and in segmental long bone defect,” Journal of Biomedical Materials Research A, vol. 96, no. 1, pp. 66–74, 2011. View at Publisher · View at Google Scholar · View at Scopus
- J. C. Reichert, D. R. Epari, M. E. Wullschleger et al., “Establishment of a preclinical ovine model for tibial segmental bone defect repair by applying bone tissue engineering strategies,” Tissue Engineering B, vol. 16, no. 1, pp. 93–104, 2010. View at Publisher · View at Google Scholar · View at Scopus
- M. J. Whitaker, R. A. Quirk, S. M. Howdle, and K. M. Shakesheff, “Growth factor release from tissue engineering scaffolds,” Journal of Pharmacy and Pharmacology, vol. 53, no. 11, pp. 1427–1437, 2001. View at Publisher · View at Google Scholar · View at Scopus
- S. E. Sakiyama-Elbert and J. A. Hubbell, “Controlled release of nerve growth factor from a heparin-containing fibrin-based cell ingrowth matrix,” Journal of Controlled Release, vol. 69, no. 1, pp. 149–158, 2000. View at Publisher · View at Google Scholar · View at Scopus
- S. W. Kang, J. S. Kim, K. S. Park et al., “Surface modification with fibrin/hyaluronic acid hydrogel on solid-free form-based scaffolds followed by BMP-2 loading to enhance bone regeneration,” Bone, vol. 48, no. 2, pp. 298–306, 2011. View at Publisher · View at Google Scholar · View at Scopus
- M. Sokolsky-Papkov, K. Agashi, A. Olaye, K. Shakesheff, and A. J. Domb, “Polymer carriers for drug delivery in tissue engineering,” Advanced Drug Delivery Reviews, vol. 59, no. 4-5, pp. 187–206, 2007. View at Publisher · View at Google Scholar · View at Scopus
- W. Chaisri, A. H. Ghassemi, W. E. Hennink, and S. Okonogi, “Enhanced gentamicin loading and release of PLGA and PLHMGA microspheres by varying the formulation parameters,” Colloids and Surfaces B, vol. 84, no. 2, pp. 508–514, 2011. View at Publisher · View at Google Scholar · View at Scopus
- E. Saito, E. E. Liao, W. W. Hu, P. H. Krebsbach, and S. J. Hollister, “Effects of designed PLLA and 50:50 PLGA scaffold architectures on bone formation in vivo,” Journal of Tissue Engineering and Regenerative Medicine. In press. View at Publisher · View at Google Scholar
- J. M. Kanczler, J. Barry, P. Ginty, S. M. Howdle, K. M. Shakesheff, and R. O. C. Oreffo, “Supercritical carbon dioxide generated vascular endothelial growth factor encapsulated poly(dl-lactic acid) scaffolds induce angiogenesis in vitro,” Biochemical and Biophysical Research Communications, vol. 352, no. 1, pp. 135–141, 2007. View at Publisher · View at Google Scholar · View at Scopus
- W. L. Murphy, M. C. Peters, D. H. Kohn, and D. J. Mooney, “Sustained release of vascular endothelial growth factor from mineralized poly(lactide-co-glycolide) scaffolds for tissue engineering,” Biomaterials, vol. 21, no. 24, pp. 2521–2527, 2000. View at Scopus
- X. B. Yang, M. J. Whitaker, W. Sebald et al., “Human osteoprogenitor bone formation using encapsulated bone morphogenetic protein 2 in porous polymer scaffolds,” Tissue Engineering, vol. 10, no. 7-8, pp. 1037–1045, 2004. View at Publisher · View at Google Scholar · View at Scopus
- S. M. Howdle, M. S. Watson, M. J. Whitaker et al., “Supercritical fluid mixing: preparation of thermally sensitive polymer composites containing bioactive materials,” Chemical Communications, no. 1, pp. 109–110, 2001. View at Scopus
- J. M. Kanczler, P. J. Ginty, L. White et al., “The effect of the delivery of vascular endothelial growth factor and bone morphogenic protein-2 to osteoprogenitor cell populations on bone formation,” Biomaterials, vol. 31, no. 6, pp. 1242–1250, 2010. View at Publisher · View at Google Scholar · View at Scopus
- M. L. Macdonald, R. E. Samuel, N. J. Shah, R. F. Padera, Y. M. Beben, and P. T. Hammond, “Tissue integration of growth factor-eluting layer-by-layer polyelectrolyte multilayer coated implants,” Biomaterials, vol. 32, no. 5, pp. 1446–1453, 2011. View at Publisher · View at Google Scholar · View at Scopus
- E. H. Sanders, R. Kloefkorn, G. L. Bowlin, D. G. Simpson, and G. E. Wnek, “Two-phase electrospinning from a single electrified jet: microencapsulation of aqueous reservoirs in poly(ethylene-co-vinyl acetate) fibers,” Macromolecules, vol. 36, no. 11, pp. 3803–3805, 2003. View at Publisher · View at Google Scholar · View at Scopus
- H. Jiang, Y. Hu, Y. Li, P. Zhao, K. Zhu, and W. Chen, “A facile technique to prepare biodegradable coaxial electrospun nanofibers for controlled release of bioactive agents,” Journal of Controlled Release, vol. 108, no. 2-3, pp. 237–243, 2005. View at Publisher · View at Google Scholar · View at Scopus
- I. C. Liao, S. Y. Chew, and K. W. Leong, “Aligned core-shell nanofibers delivering bioactive proteins,” Nanomedicine, vol. 1, no. 4, pp. 465–471, 2006. View at Publisher · View at Google Scholar · View at Scopus
- M. Hadjiargyrou and J. B. Chiu, “Enhanced composite electrospun nanofiber scaffolds for use in drug delivery,” Expert Opinion on Drug Delivery, vol. 5, no. 10, pp. 1093–1106, 2008. View at Publisher · View at Google Scholar · View at Scopus
- H. Nie, W. S. Beng, Y. C. Fu, and C. H. Wang, “Three-dimensional fibrous PLGA/HAp composite scaffold for BMP-2 delivery,” Biotechnology and Bioengineering, vol. 99, no. 1, pp. 223–234, 2008. View at Publisher · View at Google Scholar · View at Scopus
- C. Li, C. Vepari, H. J. Jin, H. J. Kim, and D. L. Kaplan, “Electrospun silk-BMP-2 scaffolds for bone tissue engineering,” Biomaterials, vol. 27, no. 16, pp. 3115–3124, 2006. View at Publisher · View at Google Scholar · View at Scopus
- Y. C. Fu, H. Nie, M. L. Ho, C. K. Wang, and C. H. Wang, “Optimized bone regeneration based on sustained release from three-dimensional fibrous PLGA/HAp composite scaffolds loaded with BMP-2,” Biotechnology and Bioengineering, vol. 99, no. 4, pp. 996–1006, 2008. View at Publisher · View at Google Scholar · View at Scopus
- J. S. Choi, K. W. Leong, and H. S. Yoo, “In vivo wound healing of diabetic ulcers using electrospun nanofibers immobilized with human epidermal growth factor (EGF),” Biomaterials, vol. 29, no. 5, pp. 587–596, 2008. View at Publisher · View at Google Scholar · View at Scopus
- S. Y. Chew, J. Wen, E. K. F. Yim, and K. W. Leong, “Sustained release of proteins from electrospun biodegradable fibers,” Biomacromolecules, vol. 6, no. 4, pp. 2017–2024, 2005. View at Publisher · View at Google Scholar · View at Scopus
- J. R. J. Paletta, K. Erffmeier, C. Theisen et al., “Influence of poly-(L-lactic acid) nanofiber functionalization on maximum load, Young's modulus, and strain of nanofiber scaffolds before and after cultivation of osteoblasts: an in vitro study,” TheScientificWorldJournal, vol. 9, pp. 1382–1393, 2009. View at Publisher · View at Google Scholar · View at Scopus
- M. D. Schofer, S. Fuchs-Winkelmann, C. Gräbedünkel et al., “Influence of poly(L-lactic acid) nanofibers and BMP-2-containing poly(L-lactic acid) nanofibers on growth and osteogenic differentiation of human mesenchymal stem cells,” TheScientificWorldJournal, vol. 8, pp. 1269–1279, 2008. View at Publisher · View at Google Scholar · View at Scopus
- L. Chen, L. Liu, C. Li, Y. Tan, and G. Zhang, “A new growth factor controlled drug release system to promote healing of bone fractures: nanospheres of recombinant human bone morphogenetic-2 and polylactic acid,” Journal of Nanoscience and Nanotechnology, vol. 11, no. 4, pp. 3107–3114, 2011. View at Publisher · View at Google Scholar · View at Scopus
- F. Ungaro, M. Biondi, I. d'Angelo et al., “Microsphere-integrated collagen scaffolds for tissue engineering: effect of microsphere formulation and scaffold properties on protein release kinetics,” Journal of Controlled Release, vol. 113, no. 2, pp. 128–136, 2006. View at Publisher · View at Google Scholar · View at Scopus
- J. Schnieders, U. Gbureck, E. Vorndran, M. Schossig, and T. Kissel, “The effect of porosity on drug release kinetics from vancomycin microsphere/calcium phosphate cement composites,” Journal of Biomedical Materials Research B, vol. 99, no. 2, pp. 391–398, 2011. View at Publisher · View at Google Scholar
- D. F. dos Santos, C. S. Bitencourt, G. M. Gelfuso et al., “Biodegradable microspheres containing leukotriene B4 and cell-free antigens from Histoplasma capsulatum activate murine bone marrow-derived macrophages,” European Journal of Pharmaceutical Sciences, vol. 44, no. 5, pp. 580–588.
- L. de Laporte, A. des Rieux, H. M. Tuinstra et al., “Vascular endothelial growth factor and fibroblast growth factor 2 delivery from spinal cord bridges to enhance angiogenesis following injury,” Journal of Biomedical Materials Research A, vol. 98, no. 3, pp. 372–382, 2011. View at Publisher · View at Google Scholar · View at Scopus
- H. Shen, X. Hu, F. Yang, J. Bei, and S. Wang, “An injectable scaffold: rhBMP-2-loaded poly(lactide-co-glycolide)/hydroxyapatite composite microspheres,” Acta Biomaterialia, vol. 6, no. 2, pp. 455–465, 2010. View at Publisher · View at Google Scholar · View at Scopus
- W. L. Lee, C. Loei, E. Widjaja, and S. C. J. Loo, “Altering the drug release profiles of double-layered ternary-phase microparticles,” Journal of Controlled Release, vol. 151, no. 3, pp. 229–238, 2011. View at Publisher · View at Google Scholar · View at Scopus
- T. Jiang, R. R. Petersen, G. Call, G. Ofek, J. Gao, and J. Q. Yao, “Development of chondroitin sulfate encapsulated PLGA microsphere delivery systems with controllable multiple burst releases for treating osteoarthritis,” Journal of Biomedical Materials Research B, vol. 97, no. 2, pp. 355–363, 2011. View at Publisher · View at Google Scholar · View at Scopus
- C. Wischke and S. P. Schwendeman, “Principles of encapsulating hydrophobic drugs in PLA/PLGA microparticles,” International Journal of Pharmaceutics, vol. 364, no. 2, pp. 298–327, 2008. View at Publisher · View at Google Scholar · View at Scopus
- K. Fu, Q. Xu, J. Czernuszka et al., “Prolonged osteogenesis from human mesenchymal stem cells implanted in immunodeficient mice by using coralline hydroxyapatite incorporating rhBMP2 microspheres,” Journal of Biomedical Materials Research A, vol. 92, no. 4, pp. 1256–1264, 2010. View at Publisher · View at Google Scholar · View at Scopus
- D. H. R. Kempen, M. J. Yaszemski, A. Heijink et al., “Non-invasive monitoring of BMP-2 retention and bone formation in composites for bone tissue engineering using SPECT/CT and scintillation probes,” Journal of Controlled Release, vol. 134, no. 3, pp. 169–176, 2009. View at Publisher · View at Google Scholar · View at Scopus
- R. Reyes, B. de la Riva, A. Delgado, A. Hernández, E. Sánchez, and C. Évora, “Effect of triple growth factor controlled delivery by a brushite-PLGA system on a bone defect,” Injury, vol. 43, no. 3, pp. 334–342, 2012. View at Publisher · View at Google Scholar · View at Scopus
- J. Rui, M. Dadsetan, M. B. Runge et al., “Controlled release of vascular endothelial growth factor using poly-lactic-co-glycolic acid microspheres: in vitro characterization and application in polycaprolactone fumarate nerve conduits,” Acta Biomaterialia, vol. 8, no. 2, pp. 511–518, 2012. View at Publisher · View at Google Scholar · View at Scopus
- W. J. King, M. W. Toepke, and W. L. Murphy, “Facile formation of dynamic hydrogel microspheres for triggered growth factor delivery,” Acta Biomaterialia, vol. 7, no. 3, pp. 975–985, 2011. View at Publisher · View at Google Scholar · View at Scopus
- Q. Sun, E. A. Silva, A. Wang et al., “Sustained release of multiple growth factors from injectable polymeric system as a novel therapeutic approach towards angiogenesis,” Pharmaceutical Research, vol. 27, no. 2, pp. 264–271, 2010. View at Publisher · View at Google Scholar · View at Scopus
- D. J. Mooney, P. M. Kaufmann, K. Sano et al., “Localized delivery of epidermal growth factor improves the survival of transplanted hepatocytes,” Biotechnology and Bioengineering, vol. 50, no. 4, pp. 422–429, 1996.
- N. H. Dormer, K. Busaidy, C. J. Berkland, and M. S. Detamore, “Osteochondral interface regeneration of rabbit mandibular condyle with bioactive signal gradients,” Journal of Oral and Maxillofacial Surgery, vol. 69, no. 6, pp. e50–e57, 2011. View at Publisher · View at Google Scholar · View at Scopus
- X. Xu, A. K. Jha, R. L. Duncan, and X. Jia, “Heparin-decorated, hyaluronic acid-based hydrogel particles for the controlled release of bone morphogenetic protein 2,” Acta Biomaterialia, vol. 7, no. 8, pp. 3050–3059, 2011. View at Publisher · View at Google Scholar · View at Scopus
- A. Hernández, E. Sánchez, I. Soriano, R. Reyes, A. Delgado, and C. Évora, “Material-related effects of BMP-2 delivery systems on bone regeneration,” Acta Biomaterialia, vol. 8, no. 2, pp. 781–791, 2012.
- S. Freitas, H. P. Merkle, and B. Gander, “Microencapsulation by solvent extraction/evaporation: reviewing the state of the art of microsphere preparation process technology,” Journal of Controlled Release, vol. 102, no. 2, pp. 313–332, 2005. View at Publisher · View at Google Scholar · View at Scopus
- M. Li, O. Rouaud, and D. Poncelet, “Microencapsulation by solvent evaporation: state of the art for process engineering approaches,” International Journal of Pharmaceutics, vol. 363, no. 1-2, pp. 26–39, 2008. View at Publisher · View at Google Scholar · View at Scopus
- J. S. Park, J. H. Lee, H. S. Shin et al., “Biodegradable polymer microspheres for controlled drug release,” Tissue Engineering and Regenerative Medicine, vol. 4, no. 3, pp. 347–359, 2007.
- S. Freiberg and X. X. Zhu, “Polymer microspheres for controlled drug release,” International Journal of Pharmaceutics, vol. 282, no. 1-2, pp. 1–18, 2004. View at Publisher · View at Google Scholar · View at Scopus
- S. Cohen, T. Yoshioka, M. Lucarelli, L. H. Hwang, and R. Langer, “Controlled delivery systems for proteins based on poly(lactic/glycolic acid) microspheres,” Pharmaceutical Research, vol. 8, no. 6, pp. 713–720, 1991. View at Scopus
- U. Bilati, E. Allémann, and E. Doelker, “Strategic approaches for overcoming peptide and protein instability within biodegradable nano- and microparticles,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 59, no. 3, pp. 375–388, 2005. View at Publisher · View at Google Scholar · View at Scopus
- P. J. Johnson, S. L. Skornia, S. E. Stabenfeldt, and R. K. Willits, “Maintaining bioactivity of NGF for controlled release from PLGA using PEG,” Journal of Biomedical Materials Research A, vol. 86, no. 2, pp. 420–427, 2008. View at Publisher · View at Google Scholar · View at Scopus
- G. Crotts and T. G. Park, “Protein delivery from poly(lactic-co-glycolic acid) biodegradable microspheres: release kinetics and stability issues,” Journal of Microencapsulation, vol. 15, no. 6, pp. 699–713, 1998. View at Scopus
- J. P. Bertram, M. F. Rauch, K. Chang, and E. B. Lavik, “Using polymer chemistry to modulate the delivery of neurotrophic factors from degradable microspheres: delivery of BDNF,” Pharmaceutical Research, vol. 27, no. 1, pp. 82–91, 2010. View at Publisher · View at Google Scholar · View at Scopus
- T. Morita, Y. Sakamura, Y. Horikiri, T. Suzuki, and H. Yoshino, “Protein encapsulation into biodegradable microspheres by a novel S/O/W emulsion method using poly(ethylene glycol) as a protein micronization adjuvant,” Journal of Controlled Release, vol. 69, no. 3, pp. 435–444, 2000. View at Publisher · View at Google Scholar · View at Scopus
- T. Suciati, D. Howard, J. Barry, N. M. Everitt, K. M. Shakesheff, and F. R. Rose, “Zonal release of proteins within tissue engineering scaffolds,” Journal of Materials Science, vol. 17, no. 11, pp. 1049–1056, 2006. View at Publisher · View at Google Scholar · View at Scopus
- Z. Cao, X. Chen, J. Yao, L. Huang, and Z. Shao, “The preparation of regenerated silk fibroin microspheres,” Soft Matter, vol. 3, no. 7, pp. 910–915, 2007. View at Publisher · View at Google Scholar · View at Scopus
- M. Ye, S. Kim, and K. Park, “Issues in long-term protein delivery using biodegradable microparticles,” Journal of Controlled Release, vol. 146, no. 2, pp. 241–260, 2010. View at Publisher · View at Google Scholar · View at Scopus
- H. Valo, L. Peltonen, S. Vehviläinen et al., “Electrospray encapsulation of hydrophilic and hydrophobic drugs in poly(L-lactic acid) nanoparticles,” Small, vol. 5, no. 15, pp. 1791–1798, 2009. View at Publisher · View at Google Scholar · View at Scopus
- B. Almería, T. M. Fahmy, and A. Gomez, “A multiplexed electrospray process for single-step synthesis of stabilized polymer particles for drug delivery,” Journal of Controlled Release, vol. 154, no. 2, pp. 203–210, 2011. View at Publisher · View at Google Scholar · View at Scopus
- F. Wang, Z. Q. Li, K. Tamama, C. K. Sen, and J. J. Guan, “Fabrication and characterization of prosurvival growth factor releasing, anisotropic scaffolds for enhanced mesenchymal stem cell survival/growth and orientation,” Biomacromolecules, vol. 10, no. 9, pp. 2609–2618, 2009. View at Publisher · View at Google Scholar · View at Scopus
- A. K. Ekaputra, G. D. Prestwich, S. M. Cool, and D. W. Hutmacher, “The three-dimensional vascularization of growth factor-releasing hybrid scaffold of poly (e{open}-caprolactone)/collagen fibers and hyaluronic acid hydrogel,” Biomaterials, vol. 32, no. 32, pp. 8108–8117, 2011. View at Publisher · View at Google Scholar · View at Scopus
- G. B. Wei, Q. M. Jin, W. V. Giannobile, and P. X. Ma, “The enhancement of osteogenesis by nano-fibrous scaffolds incorporating rhBMP-7 nanospheres,” Biomaterials, vol. 28, no. 12, pp. 2087–2096, 2007. View at Publisher · View at Google Scholar · View at Scopus
- B. Li, T. Yoshii, A. E. Hafeman, J. S. Nyman, J. C. Wenke, and S. A. Guelcher, “The effects of rhBMP-2 released from biodegradable polyurethane/microsphere composite scaffolds on new bone formation in rat femora,” Biomaterials, vol. 30, no. 35, pp. 6768–6779, 2009. View at Publisher · View at Google Scholar · View at Scopus
- T. J. Cho, L. C. Gerstenfeld, and T. A. Einhorn, “Differential temporal expression of members of the transforming growth factor β superfamily during murine fracture healing,” Journal of Bone and Mineral Research, vol. 17, no. 3, pp. 513–520, 2002. View at Scopus
- D. H. R. Kempen, M. C. Kruyt, L. Lu et al., “Effect of autologous bone marrow stromal cell seeding and bone morphogenetic protein-2 delivery on ectopic bone formation in a microsphere/poly(propylene fumarate) composite,” Tissue Engineering A, vol. 15, no. 3, pp. 587–594, 2009. View at Publisher · View at Google Scholar · View at Scopus
- B. H. Woo, B. F. Fink, R. Page et al., “Enhancement of bone growth by sustained delivery of recombinant human bone morphogenetic protein-2 in a polymeric matrix,” Pharmaceutical Research, vol. 18, no. 12, pp. 1747–1753, 2001. View at Publisher · View at Google Scholar · View at Scopus
- M. K. Nguyen and D. S. Lee, “Injectable biodegradable hydrogels,” Macromolecular Bioscience, vol. 10, no. 6, pp. 563–579, 2010. View at Publisher · View at Google Scholar · View at Scopus
- J. Zhu, “Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering,” Biomaterials, vol. 31, no. 17, pp. 4639–4656, 2010. View at Publisher · View at Google Scholar · View at Scopus
- W. Zhang, X. Wang, S. Wang et al., “The use of injectable sonication-induced silk hydrogel for VEGF165 and BMP-2 delivery for elevation of the maxillary sinus floor,” Biomaterials, vol. 32, no. 35, pp. 9415–9424, 2011. View at Publisher · View at Google Scholar
- L. Luca, A. L. Rougemont, B. H. Walpoth, R. Gurny, and O. Jordan, “The effects of carrier nature and pH on rhBMP-2-induced ectopic bone formation,” Journal of Controlled Release, vol. 147, no. 1, pp. 38–44, 2010. View at Publisher · View at Google Scholar · View at Scopus
- B. Wen, M. Karl, D. Pendrys, D. Shafer, M. Freilich, and L. Kuhn, “An evaluation of BMP-2 delivery from scaffolds with miniaturized dental implants in a novel rat mandible model,” Journal of Biomedical Materials Research B, vol. 97, no. 2, pp. 315–326, 2011. View at Publisher · View at Google Scholar · View at Scopus
- C. Hayashi, U. Hasegawa, Y. Saita et al., “Osteoblastic bone formation is induced by using nanogel-crosslinking hydrogel as novel scaffold for bone growth factor,” Journal of Cellular Physiology, vol. 220, no. 1, pp. 1–7, 2009. View at Publisher · View at Google Scholar · View at Scopus
- M. P. Lutolf, J. L. Lauer-Fields, H. G. Schmoekel et al., “Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration: engineering cell-invasion characteristics,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 9, pp. 5413–5418, 2003. View at Publisher · View at Google Scholar · View at Scopus
- S. Q. Liu, Q. Tian, L. Wang et al., “Injectable biodegradable polyethylene glycol/ RGD peptide hybrid hydrogels for in vitro chondrogenesis of human mesenchymal stern cellsa,” Macromolecular Rapid Communications, vol. 31, no. 13, pp. 1148–1154, 2010. View at Publisher · View at Google Scholar · View at Scopus
- A. Zieris, K. Chwalek, S. Prokoph et al., “Dual independent delivery of pro-angiogenic growth factors from starPEG-heparin hydrogels,” Journal of Controlled Release, vol. 156, no. 1, pp. 28–36, 2011. View at Publisher · View at Google Scholar · View at Scopus
- P. Hänseler, U. W. Jung, R. E. Jung et al., “Analysis of hydrolyzable polyethylene glycol hydrogels and deproteinized bone mineral as delivery systems for glycosylated and non-glycosylated bone morphogenetic protein-2,” Acta Biomaterialia, vol. 8, no. 1, pp. 116–123, 2012.
- X. Hao, E. A. Silva, A. Månsson-Broberg et al., “Angiogenic effects of sequential release of VEGF-A165 and PDGF-BB with alginate hydrogels after myocardial infarction,” Cardiovascular Research, vol. 75, no. 1, pp. 178–185, 2007. View at Publisher · View at Google Scholar · View at Scopus
- C. A. Simmons, E. Alsberg, S. Hsiong, W. J. Kim, and D. J. Mooney, “Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells,” Bone, vol. 35, no. 2, pp. 562–569, 2004. View at Publisher · View at Google Scholar · View at Scopus
- G. Schmidmaier, B. Wildemann, T. Gäbelein et al., “Synergistic effect of IGF-I and TGF-β1 on fracture healing in rats: single versus combined application of IGF-I and TGF-β1,” Acta Orthopaedica Scandinavica, vol. 74, no. 5, pp. 604–610, 2003. View at Publisher · View at Google Scholar · View at Scopus
- F. B. Basmanav, G. T. Kose, and V. Hasirci, “Sequential growth factor delivery from complexed microspheres for bone tissue engineering,” Biomaterials, vol. 29, no. 31, pp. 4195–4204, 2008. View at Publisher · View at Google Scholar · View at Scopus
- A. Jaklenec, A. Hinckfuss, B. Bilgen, D. M. Ciombor, R. Aaron, and E. Mathiowitz, “Sequential release of bioactive IGF-I and TGF-β1 from PLGA microsphere-based scaffolds,” Biomaterials, vol. 29, no. 10, pp. 1518–1525, 2008. View at Publisher · View at Google Scholar · View at Scopus
- D. Kaigler, P. H. Krebsbach, E. R. West, K. Horger, Y. C. Huang, and D. J. Mooney, “Endothelial cell modulation of bone marrow stromal cell osteogenic potential,” The FASEB Journal, vol. 19, no. 6, pp. 665–667, 2005. View at Publisher · View at Google Scholar · View at Scopus
- J. M. Kanczler, P. J. Ginty, J. J. A. Barry et al., “The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation,” Biomaterials, vol. 29, no. 12, pp. 1892–1900, 2008. View at Publisher · View at Google Scholar · View at Scopus
- J. L. Cleland, E. T. Duenas, A. Park et al., “Development of poly-(D,L-lactide-coglycolide) microsphere formulations containing recombinant human vascular endothelial growth factor to promote local angiogenesis,” Journal of Controlled Release, vol. 72, no. 1–3, pp. 13–24, 2001. View at Publisher · View at Google Scholar · View at Scopus
- T. W. King and C. W. Patrick, “Development and in vitro characterization of vascular endothelial growth factor (VEGF)-loaded poly(DL-lactic-co-glycolic acid)/poly(ethylene glycol) microspheres using a solid encapsulation/single emulsion/solvent extraction technique,” Journal of Biomedical Materials Research, vol. 51, no. 3, pp. 383–390, 2000.
- Z. S. Patel, S. Young, Y. Tabata, J. A. Jansen, M. E. K. Wong, and A. G. Mikos, “Dual delivery of an angiogenic and an osteogenic growth factor for bone regeneration in a critical size defect model,” Bone, vol. 43, no. 5, pp. 931–940, 2008. View at Publisher · View at Google Scholar · View at Scopus
- T. P. Richardson, M. C. Peters, A. B. Ennett, and D. J. Mooney, “Polymeric system for dual growth factor delivery,” Nature Biotechnology, vol. 19, no. 11, pp. 1029–1034, 2001. View at Publisher · View at Google Scholar · View at Scopus