Effect of Cultivation Time and Medium Condition in Production of Bacterial Cellulose Nanofiber for Urease Immobilization

Table 3

Effect of different temperatures on immobilized urease activity.

Enzyme	Immobilization matrix	Method of immobilization	Optimum temperature	Enzyme activity in optimum °C			Reference
				10°C	20°C	30°C
Urease	BCN (Medium B)	Cross-linking	50°C	98%	91%	79%	Present work
Urease	BCN (Medium A)	Cross-linking	50°C	84%	72%	61%	Present work
Urease	BCN (Medium C)	Cross-linking	50°C	88%	65%	53%	Present work
Urease	BCN (Medium D)	Cross-linking	50°C	94%	81%	73%	Present work
Urease	Electrospun polyacrylonitrile	Covalent bonding	50°C	60%	45%	30%	[28]
Urease	Nanostructured polymer	Covalent bonding	30°C	90%	—	—	[27]
Urease	Sodium alginate	Cross-linking	50°C	60	30	—	[26]
Urease	Cellulosic cotton fibers	Covalent bonding	35°C	75	—	—	[29]