Biosynthesis of Extracellular Polymeric Substances by the Marine Bacterium Saccharophagus degradans under Different Nutritional Conditions

Table 2

Experimental design (bifactorial with two replicates) to investigate the effect of nutrients and C/N ratio on EPS production.

Factor	Levels
(I) C/N ratio	3
	30
	50
	100
(II) Nutrients limitation	Limited (NL)
	Not limited (BM)

Culture mediums all together limited by reducing in 50% the basal medium salts content (K₂HPO₄, KCl, CaCl₂, MgSO₄, and FeSO₄).
Basal medium.