Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15

Table 1

Effect of metal ions on enzyme activity and stability.

Metal ions	Concentration (mM)	Residual activity (%)
		Activity	Stability
Control		100.0	100.0
CaCl2	5; 10	104.4; 153.7	135.2; 119.3
NiCl2	5; 10	101.8; 110.4	99.1; 100.5
FeSO4	5; 10	112.2; 127.9	126.3; 104.5
MgCl2	5; 10	110.9; 147.2	125.6; 113.9
CuSO4	5; 10	62.4; 50.7	53.5; 42.5
HgCl2	5; 10	41; 21	47.7; 29
MnCl2	5; 10	90.9; 85.9	83.9; 80.2
NaCl	5; 10	106.2; 121.9	101.3; 115.9
ZnSO4	5; 10	102.9; 133.6	128.4; 109
CoCl2	5; 10	125.4; 181.5	107.8; 138.2

Enzyme activity was determined at 55°C in the presence of metal ions in the reaction mixture directly and for stability enzyme was preincubated with different metal ions at 55°C for 1 h and assayed as standard assay method. The enzyme activity without incubation with metal ions was taken as 100%. Mean standard deviation for all the values is <±5.0%.