Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15
Table 2
Stability of xylanase in the presence of various organic solvents.
Organic solvents (30%)
Residual activity (%)
1 h
24 h
48 h
72 h
96 h
120 h
144 h
168 h
Methanol
−0.76
100
119.2
131.1
118.5
110.3
101.9
94.5
88.4
Isopropanol
−0.28
89
95.7
100
90.6
87
80
77.9
73.8
Ethanol
−0.24
89
93.6
97.9
90.2
89.2
85.5
80.8
78.3
Benzene
2.13
90
93
100
100
98
94
90
85.8
Cyclohexane
3.3
90
95
100
111.6
101.5
93
92
91.6
Acetone
−0.23
95.3
100.4
100.5
95.4
90
90
83
80
Butanol
−0.80
90.6
179.3
154.7
124.5
112.5
100.8
96.7
90
Toluene
2.5
96.7
190.5
170.6
149.9
100.7
99.0
90.6
90
Isooctane
2.9
97.5
137.7
116.8
102.5
100.6
92.7
90.5
87.9
Xylene
3.1
90
100
107
103
100
96
90
86
Hexane
3.6
98
179
194.7
179.8
147.5
119.9
104
95
n-Decane
5.6
99.8
189.3
219.8
208.4
160.9
137.8
107
90.0
n-Dodecane
6.0
100.8
207.5
230.8
219.5
200.6
160.9
140.0
100.0
Enzyme was preincubated with different organic solvents at a concentration of 30% (v/v) at 55°C for different time periods and assayed as standard assay method. The enzyme activity without incubation with organic solvent was taken as 100%. Mean standard deviation for all the values is <±5.0%.
