Synthesis of Poly-(R-hydroxyalkanoates) by Cupriavidus necator ATCC 17699 Using Mexican Avocado (Persea americana) Oil as a Carbon Source

Table 3

Comparison of studies reporting PHAs production in Cupriavidus necator.

Strain	Substrate	Scale	Control strategy		Biomass	PHA	PHA	Productivity	Reference
				produced	(g L^−1)	(g L^−1)	(%)	(g L^−1 h^−1)
C. necator	Plant	Flask	Batch	P(3HB)	3.6–4.3	2.9–3.4	79–81	0.04–0.05	Fukui and Doi [17]
C. necator (recombinant, Aeromonas caviae)	Plant			P(3HB-co-3HHX)	3.5–3.6	2.7–2.9	76–81	0.04
C. necator DSM 541	Fructose	Flask	Batch	P(3HB)	3.4	n.a	55	n.a	Dennis et al. [18]
	Palmitate			P(3HB-co-3HV-co-3HHX)	0.51	n.a	58	n.a
	Oleate			P(3HB-co-3HHX)	1.44	n.a	57	n.a
C. necator	Centrifuged fermented organic waste	Flask	Batch	P(3HB-co-3HV)	2.77	1.13	40.0	0.025	Ganzeveld et al., [19]
C. necator	Bagasse hydrolysate	Flask	Batch	n.a	6	3.9	65	0.08	Yu and Stahl [12]
C. necator	Palm oil	Flask	Batch	P(3HB-co-3HV)	3.6	2.66	74	n.a	Liu et al. [20]
C. necator		Flask	Fed batch	P(3PHB)	5,25	4.04	76.87	0.081	This study
	Fructose, avocado oil			P(3HB-co-3HV)	4.45–4.91	2.64–3.48	59–70	0.053–0.07

PHB, 3 hydroxybutyrate; 3HV, 3 hydroxyvalerate; 3HHX, 3-hydroxyhexanoate. oil, corn oil and palm oil tested once at a time. experiments. n.a: Not available.