Peptide–Polymer Conjugates: A Promising Therapeutic Solution for Drug-Resistant Bacteria

Figure 6

Cell viability of antigen presenting cells and B16F10 melanoma cells after exposure to melittin and α-melittin-NPs (nanoparticles). Real-time and dynamic imaging of (a) bone marrow-derived dendritic cells (BMDC), (b) bone marrow-derived macrophages (BMDMs), and (c) B16F10 cells after incubations with free melittin (5 μM) and α-melittin-NP (10 μM). Green: BMDC, magenta: BMDM, cyan: B16F10. Red indicates PI. All scale bars represent 10 μm. (d) Evaluation of cellular-binding ability by analyzing the MFI of FITC-α-melittin-NPs in B16F10 cells, BMDCs, and BMDMs (n =3 per group). Incubation time: 3 hours. MFI: mean fluorescent intensity. (e) Representative immunofluorescence imaging of cellular binding of FITC-α-melittin-NPs (10 μM) to B16F10 cells, BMDCs, and BMDMs. Incubation time: 3 hours. Blue: DAPI, green: FITC-α-melittin-NPs, red: membrane-targeted tdTomato. (Scale bar: 5 μm.) Reprinted with permission from ref [92]. Copyright 2020 Springer Nature.

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