The Reliability of a Novel Automated System for ANA Immunofluorescence Analysis in Daily Clinical Practice

Table 2

Visual versus automated detected titres of antinuclear antibodies by indirect immunofluorescence. Crosstabulation of antinuclear antibodies (ANA) titres by indirect immunofluorescence (IIF) for 89 serum samples that revealed a positive result by visual interpretation of the ANA-IIF-assay 2; concordant results are shown in bold (NEG: negative; AMB: ambiguous).

Visual interpretation (gold standard)	Automated interpretation, n
	NEG	AMB	1 : 80	1 : 160	1 : 320	1 : 640	1 : 1280	1 : 2560	1 : 5120	1 : 10240	1 : 20480	Total
1 : 80	1	15	16	1								33
1 : 160		2	2	10	1							15
1 : 320		1	1	1	6							9
1 : 640		3	1	1	1	5						11
1 : 1280					1		6	1				8
1 : 2560								7				7
1 : 5120								1	2			3
1 : 10240										1		1
1 : 20480										1	1	2
Total	1	21	20	13	9	5	6	9	2	2	1	89