Vital Autofluorescence: Application to the Study of Plant Living Cells
Figure 3
The images and fluorescence spectra of the vegetative microspores of horsetail Equisetum arvense. Sources: [8, 42] and nonpublished data of author. Upper side: Luminescence microscopy (left). Views (1–4) under the luminescence microscope Leica 6000. Microspectrofluorimetry. The fluorescence spectrum (middle) and the emission intensity distribution in form of numerical data or histogram (right). The fluorescence intensity (I) on histograms measured by dual-wavelength microspectrofluorimeter MSF-2 shows as I 520 and I 680, respectively, the emission intensity in the green region with maximum 520 nm and red region with a maximum 680 nm. Lower side: Laser-scanning confocal microscopy. Images 5–7 under laser-scanning confocal microscope LSM 510 NLO (5–moistured spore) and Carl Zeiss (6 and 7 developing spore that put off the blue-fluoresced rigid cover-envelope and then divided, resp.). On the scheme of optical image slice under laser-scanning confocal microscope (at the middle of the picture numbers mean cellular parts, from which the appropriate fluorescence spectra have been received with laser-scanning confocal microscope Leica TCS SP-5. By numerals exine (1), its inner layer intine with plasmic membrane (2) and chloroplast (3) are marked. Excitation for the luminescence microscope or microspectrofluorimeter was 420 nm and for laser-scanning confocal microscopy—405 nm laser.
