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International Journal of Spectroscopy
Volume 2012 (2012), Article ID 176851, 6 pages
http://dx.doi.org/10.1155/2012/176851
Research Article

Detection of Bacillus anthracis Spores Using Peptide Functionalized SERS-Active Substrates

Real-Time Analyzers Inc., Unit 8, 362 Industrial Park Road, Middletown, CT 06457, USA

Received 22 February 2012; Accepted 27 August 2012

Academic Editor: Kevin Spencer

Copyright © 2012 Atanu Sengupta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. G. Hess, “Biosecurity: an evolving challenge,” Chemical & Engineering News, vol. 90, no. 7, pp. 30–32, 2012.
  2. C. A. Bell, J. R. Uhl, T. L. Hadfield et al., “Detection of Bacillus anthracis DNA by light cycler PCR,” Journal of Clinical Microbiology, vol. 40, no. 8, pp. 2897–2902, 2002. View at Publisher · View at Google Scholar · View at Scopus
  3. R. Mabry, K. Brasky, R. Geiger et al., “Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays,” Clinical and Vaccine Immunology, vol. 13, no. 6, pp. 671–677, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. S. Tang, M. Moayeri, Z. Chen et al., “Detection of anthrax toxin by an ultrasensitive immunoassay using europium nanoparticles,” Clinical and Vaccine Immunology, vol. 16, no. 3, pp. 408–413, 2009. View at Publisher · View at Google Scholar · View at Scopus
  5. A. Thayer, “Homeland security: postal service readies defense—team will install PCR-based systems to detect biohazards in mail facilities,” Chemical & Engineering News, vol. 81, no. 21, article 7, 2003.
  6. T. V. Inglesby, T. O'Toole, D.A. Henderson, et al., “Anthrax as a biological weapon: updated recommendations for management,” The Journal of the American Medical Association, vol. 287, no. 17, pp. 2236–2252, 2002. View at Publisher · View at Google Scholar
  7. M. Kellogg, M. Hollis, C. Gauthier, and T. O'Brien, “Detection of biological agents used for terrorism: are we ready?” Clinical Chemistry, vol. 56, no. 1, pp. 10–15, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. R. Nudelman, B. V. Bronk, and S. Efrima, “Fluorescence emission derived from dipicolinic acid, its sodium, and its calcium salts,” Applied Spectroscopy, vol. 54, no. 3, pp. 445–449, 2000. View at Scopus
  9. P. M. Pellegrino, N. F. Fell Jr., and J. B. Gillespie, “Enhanced spore detection using dipicolinate extraction techniques,” Analytica Chimica Acta, vol. 455, no. 2, pp. 167–177, 2002. View at Publisher · View at Google Scholar · View at Scopus
  10. Y. Hathout, B. Setlow, R. M. Cabrera-Martinez, C. Fenselau, and P. Setlow, “Small, acid-soluble proteins as biomarkers in mass spectrometry analysis of bacillus spores,” Applied and Environmental Microbiology, vol. 69, no. 2, pp. 1100–1107, 2003. View at Publisher · View at Google Scholar · View at Scopus
  11. D. Naumann, “Infrared and NIR Raman spectroscopy in medical microbiology,” in Infrared Spectroscopy: New Tool in Medicine, vol. 3257 of Proceedings of SPIE, pp. 245–257, San Jose, Calif, USA, January 1998. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Farquharson, L. Grigely, V. Khitrov, W. W. Smith, J. F. Sperry, and G. Fenerty, “Detecting Bacillus cereus spores on a mail sorting system using Raman spectroscopy,” Journal of Raman Spectroscopy, vol. 35, no. 1, pp. 82–86, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. C. W. Garner, P. J. Treado, T. M. Jochem, and G. R. Gilbert, “Demonstration of a robot-based Raman spectroscopy detector for the identification of CBE threat agents,” DTIC (Army Report), 2006.
  14. S. Farquharson and W. W. Smith, “Differentiating bacterial spores from hoax materials by Raman spectroscopy,” in Chemical and Biological Point Sensors for Homeland Defense, vol. 5269 of Proceedings of SPIE, pp. 9–15, Providence, RI, USA, October 2003. View at Publisher · View at Google Scholar · View at Scopus
  15. K. Maquelin, L. P. Choo-Smith, T. van Vreeswijk et al., “Raman spectroscopic method for identification of clinically relevant microorganisms growing on solid culture medium,” Analytical Chemistry, vol. 72, no. 1, pp. 12–19, 2000. View at Publisher · View at Google Scholar · View at Scopus
  16. C. Shende, F. Inscore, A. Sengupta, and S. Farquharson, “Surface-enhanced Raman spectroscopy: theory and application to the analysis of Chlorpyrifos in orange juice,” in Applications of Vibrational Spectroscopy in Food Science, E. C. Y. Li-Chan, P. R. Griffiths, and J. M. Chalmers, Eds., pp. 195–209, John Wiley & Sons, West Sussex, UK, 2010.
  17. S. Farquharson, W. W. Smith, S. Elliott, and J. F. Sperry, “Rapid biological agent identification by surface-enhanced Raman spectroscopy,” in Air Monitoring and Detection of Chemical and Biological Agents II, vol. 3855 of Proceedings of SPIE, pp. 110–116, September 1999. View at Scopus
  18. W. G. Murrell, “Chemical composition of spores and spore structures,” in The Bacterial Spore, G. W. Gould and A. Hurst, Eds., pp. 215–273, Academic Press, London, UK, 1969.
  19. W. H. Nelson, R. Dasari, M. Feld, and J. F. Sperry, “Intensities of calcium dipicolinate and Bacillus subtilis spore Raman spectra excited with 244 nm light,” Applied Spectroscopy, vol. 58, no. 12, pp. 1408–1412, 2004. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Farquharson and F. E. Inscore, “Detection of invisible bacilli spores on surfaces using a portable SERS-based analyzer,” International Journal of High Speed Electronics and Systems, vol. 18, no. 2, pp. 407–416, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. S. Farquharson, F. E. Inscore, A. D. Gift, and C. Shende, “Method for effecting the rapid release of a signature chemical from bacterial endospores, and for detection thereof,” U.S. Patent 7, 713, 914, 2010.
  22. S. Farquharson, C. Shende, A. Gift, and F. Inscore, “Detection of bacillus spores by surface-enhanced Raman spectroscopy,” in Bioterrorism, S. S. Morse, Ed., 2012.
  23. E. Ghiamati, R. S. Manoharan, W. H. Nelson, and J. F. Sperry, “UV resonance Raman spectra of bacillus spores,” Applied Spectroscopy, vol. 46, no. 2, pp. 357–364, 1992. View at Publisher · View at Google Scholar
  24. S. Farquharson, Y. H. Lee, and C. Nelson, “Material for SERS and SERS sensors and method for preparing the same,” U.S. Patent 6, 623, 977, 2003.
  25. S. Farquharson, A. D. Gift, F. E. Inscore, and C. S. Shende, “SERS method and apparatus for rapid extraction and analysis of drugs in saliva,” U.S. Patent 7, 393, 691, 2008.
  26. W. H. Woodruff, T. G. Spiro, and C. Gilvarg, “Raman spectroscopy in vivo: evidence on the structure of dipicolinate in intact spores of Bacillus megaterium,” Biochemical and Biophysical Research Communications, vol. 58, no. 1, pp. 197–203, 1974. View at Scopus
  27. J. G. Grasselli, M. K. Snavely, and B. J. Bulkin, Chemical Applications of Raman Spectroscopy, chapter 5, John Wiley & Sons, New York, NY, USA, 1981.
  28. J. Bandekar, “Amide modes and protein conformation,” Biochimica et Biophysica Acta, vol. 1120, no. 2, pp. 123–143, 1992. View at Publisher · View at Google Scholar · View at Scopus
  29. J. C. Austin, T. Jordan, and T. G. Spiro, “UVRR studies of proteins and related compounds,” in Biomolecular Spectroscopy, R. J. H. Clark and R. E. Hester, Eds., vol. 21, John Wiley & Sons, New York, NY, USA, 1993.
  30. C. W. Garner Jr., J. S. Maier, M. P. Nelson et al., “Method for detection of pathogenic microorganisms,” US Patent 6, 917, 423, 2005.
  31. R. M. Jarvis, A. Brooker, and R. Goodacre, “Surface-enhanced Raman spectroscopy for bacterial discrimination utilizing a scanning electron microscope with a Raman spectroscopy interface,” Analytical Chemistry, vol. 76, no. 17, pp. 5198–5202, 2004. View at Publisher · View at Google Scholar · View at Scopus
  32. R. M. Jarvis and R. Goodacre, “Discrimination of bacteria using surface-enhancedRaman spectroscopy,” Analytical Chemistry, vol. 76, no. 1, pp. 40–47, 2004. View at Publisher · View at Google Scholar
  33. W. R. Premasiri, D. T. Moir, M. S. Klempner, N. Krieger, G. Jones II, and L. D. Ziegler, “Characterization of the surface enhanced Raman scattering (SERS) of bacteria,” Journal of Physical Chemistry B, vol. 109, no. 1, pp. 312–320, 2005. View at Publisher · View at Google Scholar · View at Scopus
  34. J. A. Guicheteau, K. Gonser, and S. D. Christesen, “Raman and surface enhanced Raman of biological material,” DTIC, Report No. ADA433013, 2004.