Protein Interactions Investigated by the Raman Spectroscopy for Biosensor Applications

Figure 4

The Raman spectra of (a) consecutively adsorbed SpA and IgG on SAM-Au surface and (b) SpA + IgG interacting molecules adsorbed from SpA and IgG mixed solution on SAM-Au surface. Conditions of spectra recording: time acquisition 300 s, wavelength 785 nm, nominal power laser 10 mW, optical microscope objective 100x.