Biomanufacturing versus Superficial Cell Seeding: Simulation of Chondrocyte Proliferation in a Cylindrical Cartilage Scaffold

Table 1

Properties and parameters for cellular, metabolic, cell growth kinetics and scaffold.

Property	Value	Reference
Cell density (𝜌𝑐)	182 kgm^−3	Bush and Hall, 2001 [10]
Glucose effective diffusivity in cell colony (𝐷^𝑐eff)	1.0×10^−10 m^2s^−1	Galban and Locke, 1999 [9]
Glucose effective diffusivity in the nutrient solution (𝐷^𝑓eff)	1.0×10^−9 m^2s^−1	Galban and Locke, 1999 [9]
Glucose partition coefficient (𝜆)	0.1	Galban and Locke, 1999 [9]
Cellular maximum consumption rate (𝐾)	0.03 s^−1	Galban and Locke, 1999 [9]
Initial porosity of the scaffold (𝜀)	0.92–0.96	Freed et al., 1994 [7]
Cell seeding efficiency (𝜂𝑐)	0.60	Freed et al., 1994 [7]
Initial population of seeded cells (𝑃𝑐0)	4×10^6 cells	Freed et al., 1994 [7]
Initial volumetric fraction of seeded cells (𝜀𝑐)	0.00548	Freed et al., 1994 [7]
Cell growth rate coefficient (𝑘𝑔)	1.6×10^−5 s^−1	Chung et al., 2006 [5]
Cell death rate coefficient (𝑘𝑎)	3.3×10^−7 s^−1	Chung et al., 2006 [5]
Contois saturation coefficient (𝑘𝑐)	1.54	Galban and Locke, 1999 [9]
Random walk coefficient of cells (𝐷cell)	1.7×10^−14 m^2s^−1	Barocas et al., 1995 [11]