Expression and Function of Ephrin-B1 and Its Cognate Receptor EphB2 in Human Abdominal Aortic Aneurysm
Figure 5
Modulatory effects of ephrin-B1 and EphB2 on transmigration of human PBMC by SDF-1. Boyden chamber assay was performed. (a) Effect of ephrin-B1 or EphB2 on spontaneous transmigration. The number of cells migrating into the well without SDF-1 was considered spontaneous transmigration. (b) Effect of ephrin-B1 or EphB2 on SDF-1-induced transmigration. With SDF-1, the increased number of cells migrating into the well was due to SDF-1-dependent transmigration. The level of PBMC migration with IgG-Fc used as control, which was 3.6% (a) and 18.5% (b) of inputs (1 × 105 cells), was set at 100% for analysing the effect of ephrin-B1 or EphB2. Ephrin-B1, ephrin-B1-IgG-Fc; EphB2, EphB2-IgG-Fc. The results are mean ± SEM () and were analysed by one factor ANOVA and Dunnett’s test compared with IgG-Fc (control).
