Phospholipase A2 Mediates Apolipoprotein-Independent Uptake of Chylomicron Remnant-Like Particles by Human Macrophages
Figure 4
Role of proteoglycan-mediated bridging in macrophage lipid accumulation. HMDM were incubated 1 h in serum-free medium before treatment with 33 U/mL heparinase III (Hep III) or 33 U/mL heparinase I (Hep I) in combination with 50 mM Na chlorate. Both untreated and treated macrophages were then incubated 24 h with 80 μg cholesterol/mL CRLPw/o. Treatments were continued during the incubations. Macrophage triacylglycerol (TG) and total cholesterol (TCH) contents are reported as nmol/mg protein (means ± SD, n = 4). *P < 0.005 versus control.
