Phospholipase A2 Mediates Apolipoprotein-Independent Uptake of Chylomicron Remnant-Like Particles by Human Macrophages
Figure 6
Role of macrophage lipase activities in TG synthesis. HMDM were preincubated for 2 h with or without 10 μM MJ33, 5 μM and 10 μM MAFP [36], 5 μM HELSS, and 2 μM orlistat. Incubation was then continued for 5 h in presence of CRLPw/o (80 μg cholesterol/mL) in the presence of [3H]glycerol (4 μCi/mL, 20 μM)to evaluate the incorporation of [3H]glycerol into TG. Radioactivity associated with triacylglycerol ([3H]TG) is reported as nmol/h/mg protein (n = 3). *P < 0.05 versus control.
