Selective Gene Expression Analysis of Muscular and Vascular Components in Hearts Using Laser Microdissection Method

Figure 1

(A) Representative microphotographs demonstrating the sampling of vascular area of the myocardial section obtained from SHR-SP using LMD method. (a) Laser cut lines (red lines) were placed on the outside border of the medial smooth muscle layer. (b) Myocardial section after vascular samples were cut off by laser dissection shown. (c) After laser dissection, isolated vascular fragments (red arrows) fell down into the caps of Eppendorf tubes containing TRIzol reagent. The vascular fragments were being lysed in the TRIzol reagent. (B) For sampling myocardial area, laser cut lines were placed not to include microscopically visible vasculatures and infiltrating cells in the area of interest.