The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation

Figure 2

Generation of reactive oxygen species after coculture of CRLP with primary human blood monocytes. Monocytes were preloaded with dihydrorhodamine 1, 2, 3 before incubation with CRLPs, oxCRLPs, or pCRLPs and measurement of fluorescence (ROS production) at increasing timepoints. (a, b, c) Time course of ROS production (fluorescence) 7.5 μg/mL, 15 μg/mL and 30 μg/mL cholesterol, respectively. (d, e, f) area under curve analysis (Prism) 7.5 μg/mL, 15 μg/mL and 30 μg/mL cholesterol respectively. 𝑛=5 monocyte isolations. *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001 paired t-test.

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