Myosin VI and Associated Proteins Are Expressed in Human Macrophages but Do Not Play a Role in Foam Cell Formation in THP-1 Cells

Figure 9

Inhibition of the expression of mRNA and protein for Myo6 and related proteins by siRNA. THP-1 macrophages were transfected with siRNA targeting Myo6, Dab2, AP-2α2, GIPC1 or a nonsilencing scrambled siRNA sequence (control) using HiPerFect transfection reagent. Cells were lysed at 24 h–96 h, and mRNA transcript levels for (a) Myo6, (b) Dab2, (c) AP-2α2, and (d) GIPC were determined by qPCR, and the expression of protein for (e) Myo6 and (f) Dab2 was assessed by immunoblotting. Band density was quantified using Quantity One software (Biorad), and results are expressed as % control value. +, siRNA; − scrambled siRNA. Data shown are the mean from 3 separate experiments, and error bars show the SEM. Significance limits, *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001, ****𝑃<0.0001 versus control; ^#𝑃<0.05, ^##𝑃<0.01 versus 96 h (one-way ANOVA).

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