Myosin VI and Associated Proteins Are Expressed in Human Macrophages but Do Not Play a Role in Foam Cell Formation in THP-1 Cells

Table 1

Primers, annealing temperatures, and product sizes for PCR. β2 mg, β2 microglobulin.

Target gene	Forward primer sequence (5′-3′)	Reverse primer sequence (5′-3′)	Annealing temp (°C)		Product size (bp)
			−β2 mg	+β2 mg
AP-2α2	AGCACAGAAGAACCCAGAAGAG	AGCAGTCTCAGCAGTTTGACAG	60.3	58.5	153
β-actin	AGAAAATCTGGCACCACACC	GGGGTGTTGAAGGTCTCAAA	57.3	—	142
β2-microglobulin	GTGCTCGCGCTACTCTCTCT	TCAATGTCGGATGGATGAAA	57.0	—	143
Dab2	GCAGACTTCTTCTGGGACTTTG	GTAACTGGCAGGGAAACTTGTC	60.3	58.5	165
GAPDH	AGAACATCATCCCTGCCTCTACT	GATGTCATCATATTTGGCAGGTT	56.5	—	164
GIPC1	GATGACCTGCTGGAGAGTTACA	CCAGACGTCAAAGACGAACTC	60.1	58.5	162
Myo6	GGTTTGGATGATGAAGAAAAGC	CAAACCCAGTAATTCAGCACAA	56.5	56.5	162
Myo6; (small insert)	GCAGCTTGCAGAGAAGAATTT	CTGAGGGTCTTTGTACTGGT	56.6	—	264
Myo6; (large insert)	GTTCTGGAGCAGGAGCGC	AAATTCTTCTCTGCAAGCTGC	58.2	—	312
Ribosomal protein L13a	CCTGGTCTGAGCCCAATAAA	CTTGCTCCCAGCTTCCTATG	58.0	—	144