Inhibition of Angiogenesis In Vitro by Chebulagic Acid: A COX-LOX Dual Inhibitor
Figure 3
Effect of CA on the expression of VEGF. HUVECs were maintained in culture in MCDB 131 medium containing 10% FBS supplemented with 25 μM CA for 48 hours. (a) The level of VEGF secreted into the medium was estimated using anti-VEGF antibody. Values given are the average of duplicate analysis of 5 experiments ± SEM (). (b) Western blot to analyze the production of VEGF by control cells (C) and chebulagic acid treated cells (CA) at 48 hours. The intensity of the immunoblotted bands was measured, normalised to intensity of internal control actin, and expressed in intensity units/mm2.
