Inhibition of Angiogenesis In Vitro by Chebulagic Acid: A COX-LOX Dual Inhibitor
Figure 4
HUVECs maintained in culture in MCDB 131 containing 10% FBS for 48 hours were treated with 25 μM CA. The level of MMP 2 and MMP 9 in the medium was estimated by ELISA using anti-MMP 2 (1 : 1000) (a) and MMP 9 (1 : 1000) (b) antibody, respectively. Values given are the average of duplicate analysis of 5 experiments ± SEM ().