Chronic NaHS Treatment Is Vasoprotective in High-Fat-Fed ApoE^−/− Mice

Figure 4

Superoxide generation in aorta from WT control (open bar) and fat-fed ApoE^−/− mice (shaded bar) treated with either NaHS (10 or 100 μmol/kg/day) or PPG (30 mg/kg/day). Vascular superoxide generation was significantly increased in fat-fed ApoE^−/− mice (^#𝑃<0.05, 𝑡-test, WT versus ApoE^−/−, 𝑛=7). Vascular superoxide production was significantly impaired in the groups treated with either 10 or 100 μmol/kg/day NaHS. (^∗𝑃<0.05, ANOVA, versus untreated group, 𝑛=7). PPG treatment had no effect. The flavoprotein inhibitor DPI, used to inhibit NADPH oxidase, virtually abolished the vascular superoxide signal (black bars).