Digestive Enzymes in the Crayfish Cherax albidus: Polymorphism and Partial Characterization
Figure 6
(a) Substrate SDS-PAGE showing amylolytic activity bands of juvenile Cherax albidus. (b) Substrate native-PAGE showing lipase activity bands. (c) Substrate SDS-PAGE showing protease activity bands. (d) Substrate SDS-PAGE showing protease activity bands (Ctrl) and their inhibition by specific inhibitors for trypsin (TLCK), chymotrypsin (TPCK), serine proteases (SBTI and PMSF), and aspartic proteases (pepstatin A). Each zymogram analysis was performed three times on gastric juice (GJ), hepatopancreas (H), and intestine (I) of three independent samples (). MW standards are reported on the right. The arrows indicate the active bands and their correspondent apparent molecular masses.
