Pathogens Associated with Sugarcane Borers, Diatraea spp. (Lepidoptera: Crambidae): A Review

Table 4

Entomopathogenic-nematode bioassays on Diatraea spp.

Host specie	Pathogen	Bioassay	Result	Reference
	Steinernema feltiae [Neoaplectana carpocapsae]		100% mortality with 5000 nematodes/larvae
Diatraea saccharalis	Heterorhabditis heliothidis	Several concentrations for all species	100% mortality with 5000 nematodes/larvae	Sosa et al., 1993 [42]
	S. glaseri		30% mortality with 5000 nematodes/larvae
Diatraea saccharalis	S. feltiae; S. rarum; Heterorhabditis; bacteriophora	500 IJs/5 larvae in Petri dishes with two moistened filter papers disks	Adults and IJs produced. Larval mortality >90%	De Doucet et al., 1999 [43]
Diatraea saccharalis	H. bacteriophora JPM4 isolated from soil in Larvae MG, Brazil	Larvae were exposed to 25 ± 5 IJs/100 𝜇L for 48 h in sand-filled Petri dishes	100% larval mortality within 5 days. LT50 2.1 d and LT95 4.4 d	Molina et al., 2007 [44]
Diatraea saccharalis	Heterorhabditis bacteriophora	500 mL/L applied to larvae	93% of larvae mortality	Aguila et al., 2008 [45]