Review Article

Pathogens Associated with Sugarcane Borers, Diatraea spp. (Lepidoptera: Crambidae): A Review

Table 6

Entomopathogenic-virus bioassays on Diatraea spp.

Host speciesPathogenBioassay conditionsResultReference

D. grandiosellaAutographa californica
MNPV (AcMNPV)
4 and 7 day-old larvae were fed strips of corn leaf soaked in different dilutions of virus. DL50: 1.32 103 PIB*/larvae (for 4-day-old larvae)
DL50: 1.32 104 PIB/larvae (for 7-day-old larvae).
Davis and Sikorowsy, 1978 [54]
D. saccharalisDiatraea saccharalis Granulosis Virus
(DsGV)
3rd instar larvae feeding individually on small artificial diet discs treated with 2.7 μL of virus dilutions. LD50: 42 PIB/larva at 26˚C
LT50: From 29 to 63 for 107 and102 PIB/larvae, respectively.
Lastra and Gómez
et al., 1983 [16]
D. saccharalisAnticarsia gemmatalis MNPV (AgMNPV)
3rd instar larvae fed with artificial diet discs treated with 5 different doses of PIB through 20 serial passages. LD50: From 7.9 105 to 5.3 102 for 1 and 20 serial passages, respectively.
Pavan and Ribeiro, 1989 [55]
D. saccharalisAgMNPV wt



AgMNPV-D10
3rd instar larvae individually fed small artificial diet discs containing 105 PIB of virus and kept individually at 10 different temperatures.LT50: From 10 to 13 for
39 and 30˚C, respectively

From 9 to 29 for 39 and 22˚C, respectively
Ribeiro and Pavan, 1994 [56]
D. saccharalisTrichoplusia ni MNPV (TnMNPV) wt


TnMNPV-D11
3rd instar larvae fed individually with small artificial diet discs containing 105 PIB of virus and kept individually at 10 different temperatures.LT50: From 9 to 16 for
39 and 24˚C, respectively


From 6 to 46 for 39 and 17˚C, respectively
Ribeiro and Pavan, 1994 [56]
D. sacccharalisAgMNPV3rd instar larvae fed on formalin free diet disc (3 mm) inoculated with 2.7 μL of the viral solution from 10 clonal isolates of the AgMNPV-Ds20 LD50: From 5.3 × 103  PIB/larvae (7A genotypic variant) to 8 × 104 PIB/larvae (33B genotypic variant)
Ribeiro et al., 1997 [57]

*PIB: Polyhedral inclusion bodies.