Studies on the Pinctada fucata BMP-2 Gene: Structural Similarity and Functional Conservation of Its Osteogenic Potential within the Animal Kingdom
Figure 2
Dose-response effect of BMP-2 on alkaline phosphatase activity. C3H10T1/2 cells were plated into 48-well tissue culture dishes and cultured for 2 days under normal conditions. The cells were then cultured for a further 10 days with the recombinant mature domain of PfBMP-2 (a) (1.0, 1.5, 2.5, and 5 μg/well) or the synthetic polypeptide of the knuckle epitope of human BMP-2 (b) (10, 40, 80, and 200 μg/well). The alkaline phosphatase in each well was then assayed and the results are presented as micromoles of p-nitrophenol (p-NP) produced per minute per milligram of protein.
