Studies on the Pinctada fucata BMP-2 Gene: Structural Similarity and Functional Conservation of Its Osteogenic Potential within the Animal Kingdom
Figure 8
Immunohistochemical staining for osteogenic markers in C3H10T1/2 cells. C3H10T1/2 cells were plated into 40 mm culture dishes for 2 days and then treated with 250 ng/mL PfBMP-2 synthetic polypeptide or 1 μg/mL synthetic polypeptide of the knuckle epitope of human BMP-2 (hBMP-2) for the indicated time periods. (a) Immunohistochemical staining of collagen type I after 7 days in culture. (b) Immunohistochemical staining of osteopontin after 13 days in culture. (c) Immunohistochemical staining of osteocalcin after 18 days in culture. Cells were photographed at 100x magnification.
