Review Article

The Human Vaginal Bacterial Biota and Bacterial Vaginosis

Figure 7

Vaginal biopsy from a subject with BV. A Gardnerella vaginalis biofilm (yellow) is detected at the edge of the vaginal epithelium (bottom) by fluorescence in situ hybridization (FISH). The yellow color is the result of using a combination of probes targeting G. vaginalis (Red), all bacteria (Eub338, green), and ,6-diamidine-2-phenylindole, dihydrochloride (DAPI, blue) which stains DNA. Note human cell nuclei in blue. The image on the right shows a vaginal epithelial cell with a cluster of G. vaginalis breaking off the epithelium and likely forming a clue cell.
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