Diagnosis of Parasitic Diseases: Old and New Approaches

Figure 3

Molecular-based assay: PCR. Example of PCR results obtained for seven monkey samples, using the TCRUZ primers. Blood samples were processed as described in Materials and Methods. The PCR products were electrophoresed in a 2% agarose gel and stained with ethidium bromide. The 168 bp band (arrow) is the expected T. cruzi-specific product. The 360 and 550 bp are also specific products resulting from amplification of two or three of the 195 bp repeats found in tandem arrays in the T. cruzi genome. Lanes 1 to 6 contain the amplification products of DNA from T. cruzi-infected monkeys; 7, blood from noninfected monkey; 8, negative control (distilled water); and M, 100 bp ladder, adapted, with permission of Comparative Medicine from [9].