Sterol Biosynthesis Pathway as Target for Anti-trypanosomatid Drugs
Figure 13
DIC microscopy ((a)-(b), left panel), fluorescence with DAPI ((a)-(b), right panel) and transmission electron microscopy (c)-(d) of treated-promastigotes to evidenciate the alterations in the cell cycle after treatment with BPQ-OH and ER-119884. (a) Control cells present a correct number of kinetoplast (white arrow), nucleus (arrowhead), and flagellum (black arrow), one of each for a unique cell. (b) After treatment, the number of these organelles is completely alterated and it is possible to find cells with one flagellum (black arrow), four kinetoplast (white arrows), and one nucleus (arrowhead). (c)-(d) Alterations in the cell cycle can also be evidenced by transmission electron microscopy with the appearance of cells with several nuclei and kinetoplasts. K, kinetoplast; N, nucleus. These images are reproduced with permission from [37] American Society for Microbiology (a), (b), (d), and from [66] Elsevier (c). Bars, m (a)-(b), and m (c)-(d).
