|
Species amplified | Sequence ( to ) | Primer /probe*name | Annealing temp. in PCR/melting temp.# (°C) | Amplicon size, base pairs | Reference(s); notes |
|
Primers used in PCR |
|
Anncaliia (Brachiola) algerae | ACTCCGGTAACGTGTGATGTG | NALGf2 | 55 | 180 | [37] |
TACAAAGCATGATCCCAGTCT | NALGR1 | | | |
|
Anncaliia (Brachiola) algerae | GCCGTTTCCGAAGTTGG | NAGf | 50 | 192 | [38] |
ATATCGACGGGACTCTCACC | NAG178r | | | |
|
Encephalitozoonidae and Ent. bieneusi | CACCAGGTTGATTCTGCCTGAC | PMP1 (V1) | 60 | Eb 250 | [17] |
CCTCTCCGGAACCAAACCTG | PMP2 | Ec 268 | |
| | Ei 270 | |
| | Eh 279** | |
|
Encephalitozoonidae and Ent. bieneusi | TGAATG(G/T)GTCCCTGT | MSP1 | 58 | Eb 508 | [22] |
TCACTCGCCGCTACT | MSP2A | Ec 289 | |
GTTCATCGCACTACT | MSP2B | Ei 305 | |
GGAATTCACACCGCCCGTC(A/G)(C/T)TAT | MSP3 | | |
CCAAGCTTATGCTTAAGT(C/T)(A/C)AA(A/G)GGGT | MSP4A | | |
CCAAGCTTATGCTTAAGTCCAGGGAG | MSP4B | | |
|
Encephalitozoonidae and Ent. bieneusi | CCAGGUTGATUCTGCCUGACG | Mic3U | 65/62 | Eb 132 | [20] |
TUACCGCGGCUGCUGGCAC | Mic421U | Ec 113 | |
AAGGAGCCTGAGAGATGGCT | Mic266 | Eh 134 | |
CAATTGCTTCACCCTAAGGTC | Eb379 | Ei 128 | |
GACCCCTTTGCACTCGCACAC | Ec378 | | |
TGCCCTCCAGTAAATCACAAC | Eh410 | | |
CCTCCAATCAATCTCGACTC | Ei395 | | |
|
Encephalitozoonidae and Ent. bieneusi | CACCAGGTTGATTCTGCC | C1 (V1) | 56 | Eb 1170 | [18] |
GTGACGGGCGGTGTCTAC | C2 | Ec 1190 | |
| | Eh 1205 | |
| | Ei 1186*** | |
|
Encephalitozoonidae | TGCAGTTAAAATGTCCGTAGT | int530f | 40 | 1000 | Didier et al., [39] |
TTTCACTCGCCGCTACTCAG | int580r | |
|
Enc. intestinalis | CACCAGGTTGATTCTGCCTGAC | V1 | 58 | 375 | [40] |
CTCGCTCCTTTACACTCGAA | Si500 | |
|
Enc. intestinalis | GGGGGTAGGAGTGTTTTTG | 3 | 65 | 930 | Schuitema et al., 1993, [41] |
CAGCAGGCTCCCTCGCCATC | 3 | |
|
Enc. intestinalis | TTTCGAGTGTAAGGAGTCGA | SINTF1 | 55 | 520 | [42, 43] |
CCGTCCTCGTTCTCCTGCCCG | SINTR | |
|
Enc. cuniculi | ATGAGAAGTGATGTGTGTGCG | ECUNF | 55 | 549 | [43, 44] |
TGCCATGCACTCACAGGCATC | ECUNR | |
|
Enc. hellem | TGAGAAGTAAGATGTTTAGCA | EHELF | 55 | 547 | [44] |
GTAAAAAGACTCTCACACTCA | EHELR | |
|
Ent. bieneusi | GAAACTTGTCCACTCCTTACG | EBIEF1 | 55 | 607 | [42] |
CCATGCACCACTCCTGCCATT | EBIER1 | |
Ent. bieneusi | CACCAGGTTGATTCTGCCTGAC | V1 | 48 | 353 | [45, 46] |
ACTCAGGTGTTATACTCACGTC | EB450 | |
|
Ent. bieneusi | CACCAGGTTGATTCTGCCTGAC | V1 | 54 | 446 | [24, 47] |
CAGCATCCACCATAGACAC | Mic3 | |
|
Ent. bieneusi | TCAGTTTTGGGTGTGGTATCGG | Eb.gc | 49 | 210 | [48] |
GCTACCCATACACACATCATTC | Eb.gt | |
|
Ent. bieneusi | GCCTGACGTAGATGCTAGTC | 2 | 55 | 1265 | [49] |
ATGGTTCTCCAACTGAAACC | 2 | |
|
Vittaforma corneae | TGAGACGTGAAGATGAGTATC | NCORF1 | 55 | 375 | Pieniazek NJ and Visvesvara GS personal communication |
| TCCCTGCCCACTGTCTCCAAT | NCORR1 | |
|
Primers and probes used in hybridization |
|
Encephalitozoon spp. | CAGGTTGATTCTGCCTGACG | FP | 63 | | Notermans et al., [50] |
ATCTCTCAGGCTCCCTCTCC | RP | | | |
|
Enc. hellem | ACT CTCACA CTC ACT TCA G | HEL878F | 54 | | [51] |
|
Ent. bieneusi | CGGTGGTGTGTGTAGGCGTGAGAGTGTATC | probe | 55 | | [52] |
| | (hybridization) | | |
|
Primers and probes used in RT-PCR or fluorogenic PCR |
|
EncephP1 | CCC TGT CCT TTG TAC ACA CCG CCC | EncephP1 | 68 | | [53] |
EcunF1 | TCC TAG TAA TAG CGG CTG ACG AA | EcunF1 | 59 | | |
EcunR2 | ACT CAG GAC TCA GAC CTT CCG A | EcunR2 | 59 | | |
EhelF1 | GAA TGA TTG AAC AAG TTA TTT TGA ATG TG | EhelF1 | 59 | | |
EhelR2 | AAC ACG AAA GAC TCA GAC CTC TCA | EhelR2 | 58 | | |
EintF1 | AAT TCC TAG TAA TAA CGA TTG AAC AAG TTG | EintF1 | 59 | | |
EintR2 | ACG AAG GAC TCA GAC CTT CCA A | EintR2 | 59 | | |
|
Ent. bieneusi | CGCTGTAGTTCCTGCAGTAAACTATGCC | FEB1 | 65 | 102 | [33, 34] |
CTTGCGAGCGTACTATCCCCAGAG | REB1 | | | |
| ACGTGGGCGGGAGAAATCTTTAGTGTTCGGG | probe | | | |
|
Enc. intestinalis | GCAAGGGAGGAATGGAACAGAACAG | FEI1 | 65 | 127 | [33] |
TTCAGAAGCCCATTACACAGC | REI1 | | | |
| CGGGCGGCACGCGCACTACGATA | probe | | | |
|
Ent. bieneusi | TGTGTAGGCGTGAGAGTGTATCTG | EbITS-89F | 60 | 103 | [54] |
CATCCAACCATCACGTACCAATC | EbITS-191R | | | |
| CACTGCACCCACATCCCTCACCCTT | EbITS-114revT | | | |
Encephalitozoon spp. | CACCAGGTTGATTCTGCCTGAC | MSP1F | 60 | | |
| CTAGTTAGGCCATTACCCTAACTACCA | Eint227R | | | |
| CTATCACTGAGCCGTCC | Eint82Trev | | | |
|
Encephalitozoon | GTCCGT TAT GCC CTG AGA T | | 60 | 268 | |
| ACA GCA GCC ATG TTA CGACT | | | | |
| GCC CGT CGC TATCTA AGA TGA CGC A | probe 1 | | | |
| TGG ACG AAG ATT GGA AGGTCT GAG TC | probe 2 | | | |
|
Primers and probes used in oligonucleotide microarray |
|
microsporidia-generic | GATTCTGCCTGACGTGGATGCTATT | Msp1 | 58 | | [55] |
| ATTCCGGAGAGGGAGCCTGAGAGAT | Msp2 | 61 | | |
| ATTGACGGAAGGACACTACCAGGA | Msp3 | 57 | | |
| GTGCGGCTTAATTTGACTCAACGCG | Msp4 | 58 | | |
|
Ent. bieneusi | ACGGCTCAGTAATGTTGCGGTAATT | Eb1 | 56 | | |
CCTATCAGCTTGTTGGTAGTGTAAA | Eb2 | 54 | | |
| TCATGAGACGTGAGTATAAGACCTG | Eb3 | 56 | | |
| ATCGAATACGTGAGAATGGCAGGAGT | Eb4 | 58 | | |
| CTAAAAGCGGAGAATAAGGCGCAAC | Eb5 | 58 | | |
| CGTTGTTCAATAGCGATGAGTTTGC | Eb6 | 56 | | |
| GGTGAAACTTAAAGCGAAATTGACGG | Eb7 | 56 | | |
| AGCCTGTGTGTGAGAATACGTGG | Eb8 | 56 | | |
|
Encephalitozoon | ACGGCTCAGTGATAGTACGATGATT | Ence1 | 56 | | |
| TATCAGCTGGTAGTTAGGGTAATGG | Ence2 | 56 | | |
| GAGTGAAACTTGAAGAGATTGACGG | Ence3 | 56 | | |
|
Enc. cuniculi | TGTGGGGTTGGCAAGTAAGTTGTGG | Ec1 | 59 | | |
| ATGAGAAGTGATGTGTGTGCGAGTG | Ec2 | 58 | | |
| GCCTGTGAGTGCATGGCATGAG | Ec3 | 59 | | |
| GGGAAACTGCAGATAGTGGTCTGC | Ec4 | 59 | | |
| GGATGTAGTGATGTGTGTGGCAGAG | Ec5 | 59 | | |
| CTGGACGGGACAGTGTGTGTTGT | Ec6 | 59 | | |
|
Enc. hellem | TAAGTTCTGGGGGTGGTAGTTTGTA | Eh1 | 56 | | |
| GCGGTTATGAGAAGTAAGATGTTTAGCA | Eh2 | 57 | | |
| CTGAAGTGAGTGTGAGAGTGTTTTTAC | Eh3 | 57 | | |
| ATTGGGAGCCTGGATGTAACTGTGG | Eh4 | 59 | | |
| GGATGTAGTTTTATTGTAGCAGAGG | Eh5 | 54 | | |
| TGGACGGGACTGTTTTAGTGTTGTC | Eh6 | 58 | | |
Enc. intestinalis | TTGACACGAGCCAAGTAAGTTGTAG | Ei1 | 56 | | |
| TTTCGAGTGTAAAGGAGTCGAGATTGA | Ei2 | 57 | | |
| GGCAGGAGAACGAGGACGGGAT | Ei3 | 60 | | |
| CAGGTAGGGGGCTAGGAGTGTTTTT | Ei4 | 59 | | |
| TATGTCCTGATGTGGATGTAAGAGG | Ei5 | 56 | | |
| TGGACGGGACTATATAGTGTTGTG | Ei6 | 56 | | |
|
microsporidia-generic | TTTMMAACGGCCATGCACCAC | MspR3 | 52 | var. | Used to label initial PCR products |
|