Detection of viral antigen spread by EGFP fluorescence. Mice ware sacrificed at day 5 p.i. Brain was removed and processed for frozen sections. A portion of sagittal brain sections was cut from the middle of the brain of infected mice. Frozen sections were processed for detection of viral antigen distribution by EGFP fluorescence. Bright EGFP fluorescence was spread throughout the brain section as showed by montage assembled from 10 serial images (a). Higher magnification image (b) from the same brain section shows the cellular distribution of EGFP fluorescence. (adapted from the wprk of [114]).
