CNS pathology of EGFP expressing MHVs. (a, b) Demyelination: luxol fast blue-stained spinal cord sections of mice infected with (a) or RSMHV (b), at day 30 post infection. Thin arrows indicate a region of demyelination, and the arrowheads indicate normal myelinated area of white matter. (c, d) Encephalitis: microglial nodules: Hematoxylin- and Eosin-stained sections from the basal forebrain of mice infected with EGFP tagged MHVs at day 7 post infection. Arrows indicate the microglial nodules (microglia with elongated nuclei) and lymphocytes in the vicinity of neurons in mice infected with (c) and RSMHV (d). (e, f) Meningitis. Hematoxylin- and eosin-stained sections from the basal forebrain of mice infected with EGFP tagged MHVs at day 7 post infection: and RSMHV. In all cases, there is a brisk leptomeningeal lymphocytic inflammatory infiltrate. An original magnification for (a, b) is 20x and (c–f) is 200x. (adapted from the work of [116]).
