| Figure 1: Representative images of double immunolabelling studies to demonstrate the infection of astrocytes, microglia, and neurons with Chlamydia pneumoniae in the AD brain. Chlamydia pneumoniae-infected cells were identified in all cases using the FITC-labelled monoclonal antibody targeting the Chlamydia LPS (Pathfinder TM; a, c, e). Astrocytes (b) and microglia (d) were identified by immunostaining using monoclonal antibodies targeting GFAP and iNOS, respectively. Neurons were identified by immunostaining with a monoclonal antibody targeting neuron-specific microtubule-associated protein (f). Images in all panels were obtained using a objective. In all panels, arrows indicate cells labelling with the Pathfinder TM and surface marker-specific monoclonal antibodies. (Reproduced from [54] with courtesy of Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI, USA, and with permission of FEMS Med Lett). |
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