Neuroinvasion in Prion Diseases: The Roles of Ascending Neural Infection and Blood Dissemination
Figure 3
Scrapie infection in lymphoreticular tissues in ARQ/ARQ Suffolk sheep. (a) PrPd accumulation is associated with both FDC and macrophages in clinically affected sheep as shown by single IHC for PrP antibody R145. Peyer’s patches of the distal ileum. (b) PrPd within TBMs is N terminally truncated whereas PrPd associated with FDC is not [52]. TBM and FDC can be distinguished in tissue sections by using N or C terminal PrP antibodies. FDCs (brown), labelled by the N terminal antibody FH11, are in light zone whereas TBMs (purple), labelled by the C terminal antibody R145, are both in dark and light zones of secondary lymphoid follicles: double IHC for FH11 (brown, DAB substrate) and R145 (purple, VIP substrate). In spleen, nerve endings located in the marginal zone ((c), IHC for PgP9.5) do not colocalised with PrPd deposition ((d), IHC for R145). (a)–(d) x20.
