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Interdisciplinary Perspectives on Infectious Diseases
Volume 2011 (2011), Article ID 462767, 10 pages
http://dx.doi.org/10.1155/2011/462767
Research Article

Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in Malaria

1Laboratoire d'Immunologie EA 2686, IMPRT-IFR 114, Faculté de Médecine Pôle Recherche, Université Lille 2, 1 Place de Verdun, 59045 Lille Cedex, France
2Laboratoire d'Immunologie et Hématologie du CHU-Cocody, Abidjan, Cote D'Ivoire
3Laboratoire de Parasitologie et de Mycologie, Institute de Biologie et Pathologie, CHRU de Lille 59037 Lille, France
4Service de Neurologie D, Hôpital Roger Salengro, 59037 Lille Cedex, France

Received 6 July 2011; Accepted 23 September 2011

Academic Editor: Jose G. Estrada-Franco

Copyright © 2011 Romuald Dassé et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Absence of acquired protective immunity in endemic areas children leads to higher susceptibility to severe malaria. To investigate the involvement of regulatory process related to self-reactivity, we evaluated potent changes in auto-antibody reactivity profiles in children and older subjects living in malaria-endemic zones comparatively to none-exposed healthy controls. Methods. Analysis of IgG self-reactive footprints was performed using Western blotting against healthy brain antigens. Plasmas of 102 malaria exposed individuals (MEIs) from endemic zone, with or without cerebral malaria (CM) were compared to plasmas from non-endemic controls (NECs). Using linear discriminant and principal component analysis, immune footprints were compared by counting the number, the presence or absence of reactive bands. We identified the most discriminant bands with respect to age and clinical status. Results. A higher number of bands were recognized by IgG auto-antibodies in MEI than in NEC. Characteristic changes in systemic self-IgG-reactive repertoire were found with antigenic bands that discriminate Plasmodium falciparum infections with or without CM according to age. 8 antigenic bands distributed in MEI compared with NEC were identified while 6 other antigenic bands were distributed within MEI according to the age and clinical status. Such distortion might be due to evolutionary processes leading to pathogenic/protective events.