ITS1 PCR-RFLP Diagnosis and Characterization of Leishmania in Clinical Samples and Strains from Cases of Human Cutaneous Leishmaniasis in States of the Mexican Southeast
Figure 1
(a) Electrophoresis run at 100 V for 30 min. (b) Electrophoresis run at 199 V for 60 min. PCR-RFLP of the ITS1 of Leishmania reference strains. Lane 1: Leishmania (V.) panamensis MHOM/CR/87/NEL3; lane 2: Leishmania (V.) panamensis MHOM/PA/72/LS9; lane 3: Leishmania (V.) guyanensis (MHOM/BR/75/M4147); lane 4: Leishmania (L.) mexicana (MHOM/MX/85/SOLIS); lane 5: Leishmania (V.) braziliensis (MHOM/BR/75/M2903); lane 6: Leishmania (L.) amazonensis (MHOM/BR/73/M2269); lane 7: MWMX174 Hae III.